茉莉酸甲酯局部诱导长白落叶松对舞毒蛾生长发育的影响

Effects of locally inducing Larix olgensis using exogenous methyl jasmonate on the growth and development of Lymantria dispar

为了解茉莉酸甲酯局部处理对林木诱导抗虫性的影响,本研究以长白落叶松幼苗为试验材料,分别用浓度为0.01、0.10和1.00 mmo L/L的茉莉酸甲酯全株或局部喷施处理,分析不同处理苗木对舞毒蛾生长和繁殖的影响。结果表明,取食3个浓度茉莉酸甲酯局部或全株处理过的长白落叶松后,舞毒蛾的3-6龄幼虫平均质量、蛹质量以及成虫产卵量与对照组相比均显著下降（P〈0.05）,幼虫存活率、化蛹率、羽化率也都低于对照组,且相同浓度的局部处理与全株处理间结果相近,无显著差异（P〉0.05）,其中0.10 mmo L/L茉莉酸甲酯局部处理诱导效果最好。本研究结果说明3个浓度茉莉酸甲酯局部和全株处理后均能不同程度诱导长白落叶松增强其对舞毒蛾生长发育的抑制能力,并且茉莉酸甲酯局部喷施长白落叶松可以达到全株喷施的诱导效果,以0.10 mmo L/L浓度的诱导效果最好。

In order to understand how local spraying of exogenous methyl jasmonate would affect the induced resistance level of trees to insects, we sprayed identical amounts of 0.01, 0. 10 and 1.00 mmol/L methyl jasmonate, respectively, on whole Larix olgensis seedlings or on specific parts of the seedlings. We analyzed the effects of the methyl jasmonate treatments on the growth and reproduction of the gypsy moth （Lymantria dispar）. The results showed that feeding gypsy moth larvae on plant sprayed either partially or entirely with these three concentrations of methyl jasmonate could decrease significantly the average larval weight of 3rd to 6th instars, the survival rate of the larvae, pupal weight, pupal and adult eclosion rates and the fecundity of adult females, compared to the control group （P 〈 0.05）. The same concentration of methyl jasmonate applied either to parts or to entire seedlings had no significant difference in the effect on the insect growth （P 〉 0.05 ）. The 0. 10 mmol/L concentration of methyl jasmonate sprayed on parts of plant had the best inductive effects. The above results illustrate that all three concentrations of methyl jasmonate applied either to parts or to entire seedlings can enhance the ability of larch to inhibit the growth and development of gypsy moth, and the partial spraying methyl jasmonate on larch seedlings can achieve the same inductive effects as spraying on the whole seedlings.