胃癌与癌旁组织中RACK1、Src和Bcl-2蛋白的表达及相关性研究

Study on expression of RACK1,Src and Bcl-2 protein and their correlation in gastric carcinoma tissues and adjacent carcinomatous tissues

目的探讨胃癌及癌旁组织中活化的蛋白激酶C受体-1(RACK1)、类固醇受体辅助活化因子(Src)和B淋巴细胞瘤-2(Bcl-2)基因表达的变化。方法收集华北理工大学附属医院2011年8月1日至2014年2月1日手术切除胃癌及癌旁组织标本各80份,采用免疫组织化学法及蛋白质印迹法(Western blotting)检测RACK1、Src及Bcl-2蛋白在胃癌及癌旁组织中的表达情况,分析其相关性并结合临床病理学资料进行统计学分析。结果免疫组织化学法与Western blotting检测显示,胃癌组织中RACK1表达阳性率及表达水平均低于癌旁组织,Src与Bcl-2表达阳性率及表达水平均高于癌旁组织,差异均有统计学意义(P<0.05)。在胃癌组织中,RACK1表达分别与Src、Bcl-2表达呈负相关(相关系数r值分别为-0.632、-0.754,P<0.01),Src与Bcl-2蛋白表达无相关性(r=0.217,P>0.05)。结论胃癌组织中RACK1表达明显降低,而Src与Bcl-2表达升高。

Objective To investigate the expression change of RACK1,Src and Bcl-2in gastric carcinoma tissue and adjacent carcinomatous tissue.Methods Eighty specimens of gastric carcinoma and adjacent carcinomatous tissues in our hospital from August 1,2011 to February 1,2014 were collected.The immunohistochemistry staining and Western blotting methods were adopted to detect the expression of RACK1,Src and Bcl-2protein in gastric carcinoma and adjacent carcinomatous tissues,and their correlation was analyzed and performed the statistical analysis by combining with the clinicopathological data.Results The immunohistochemistry staining and Western blotting detection displayed that the expression positive rate and expression level of RACK1 in gastric carcinoma tissue were obviously lower than those in the adjacent carcinomatous tissue,while the expression positive rate and expression level of Src and Bcl-2in gastric carcinoma tissue were obviously higher than those in the adjacent carcinomatous tissue,the differences were statistically significant(P<0.05).The RACK1 expression in gastric carcinoma tissue was negatively correlated with the Src and Bcl-2expression(r=-0.632,-0.754,P<0.01),while Src had no obvious correlation with Bcl-2protein(r=0.217,P>0.05).Conclusion The expression of RACK1 in gastric carcinoma tissue is significantly decreased,while the expressions of Src and Bcl-2are increased.