苦参凝胶治疗阴道假丝酵母病的药效学研究

Pharmacodynamic study of Sophora flavescens alkaloid gels for treatment of vaginal candidiasis

目的在动物模型上进行苦参凝胶治疗阴道假丝酵母病的药效学研究。方法建立阴道假丝酵母病BALB/c小鼠模型,分成模型组、空白凝胶组、苦参凝胶组和酮康唑乳膏组,每天阴道内给药1次,连续7 d,于治疗前和给药后第4、7天取阴道分泌物涂片染色观察。阴道冲洗液在体外培养白色假丝酶母。第7次给药后处死动物,取阴道组织进行病理检查。结果与模型组和空白凝胶组比较,苦参凝胶和酮康唑乳膏两组均可使阴道白色假丝酵母菌落数显著减少。苦参凝胶可增加阴道有益菌-乳杆菌数量,而酮康唑不增加。阴道冲洗液体外培养和计数结果表明,苦参凝胶和酮康唑乳膏可抑制白色假丝酵母生长(P<0.01)。苦参凝胶还能抑制阴道黏膜水肿及炎性增生。结论苦参凝胶通过抑制白色假丝酵母生长、促进乳杆菌增殖、抑制阴道黏膜炎性增生等机制治疗白色假丝酵母阴道炎。

Objective To explore the pharmacodynamic effect of Sophora flavescens alkaloid（SFA）gels for the treatment of vaginal candidiasis on animal models. Method The BALB/c mice models having vaginal candidiasis were prepared. The animals were divided into four groups：the model group,the blank gel group,the SFA gel group,and the ketoconazole cream group. The regimens were administered to the animals once a day for seven days. Before treatment and after the fourth and seventh treatments,the vaginal smears were collected and Gram-stained. The vaginal washings were cultured to detect Candida albicans. On day 7,the mice were sacrificed and the vaginal tissues were excised for pathological detection. Results Compared with the model and the blank gel groups,the SFA gel and ketoconazole cream groups reduced the C. albicans colonies in the vagina. Moreover,the SFA gels increased the number of Lactobacillus,the helpful bacteria in vagina but the ketoconazole creams did not. The SFA gels and ketoconazole creams inhibited the growth of C. albicans according to the investigation of in vitro culture of vaginal washings（P0.01）. Furthermore,the SFA gels inhibited the inflammatory hyperplasia and edema. Conclusion The therapeutic action of SFA gels on vaginal candidiasis is achieved by inhibiting the growth of candidiasis,promoting lactobacillus growth and inhibiting the inflammatory hyperplasia.