改良多囊卵巢综合征大鼠模型卵泡颗粒细胞中凋亡调控蛋白Bcl-2 Bax表达的研究

Research on the Expression of Apoptosis Regulatory Proteins Bcl-2,Bax in Ovarian Follicle Granulosa Cells of Improved PCOS Rat Model

目的:探讨凋亡调控蛋白Bcl-2和Bax在改良多囊卵巢综合征(PCOS)大鼠模型卵巢的卵泡中颗粒细胞中的表达。方法:分别在来曲唑(Letrozole)和脱氢表雄酮(DHEA)造模基础上加用高脂乳剂,建立改良PCOS动物模型,结合阴道脱落细胞涂片、大鼠血清性激素水平、处死时体重增加幅度和卵巢形态学的改变进行模型验证,选用免疫组织化学法检测大鼠卵巢组织Bcl-2、Bax的表达。结果:模型建立成功。免疫组织化学结果显示高脂模型组、Letrozole模型组、Letrozole改良组、DHEA模型组和DHEA改良组Bax平均灰度均值均高于空白对照组,Bcl-2平均灰度均值均低于空白对照组,差异无统计学意义。结论:Bcl-2在卵巢的卵泡颗粒细胞中表达下调,Bax表达上调,推测两者表达改变可能是导致改良PCOS模型大鼠卵巢中颗粒细胞凋亡增多的关键因素。

Objective： To study the expression of the apoptosis of modulin Bcl-2,Bax in follicle granulosa cells of the improved Polycystic Ovary Syndrome（ PCOS） rat model ovary. Methods： The obesity PCOS rat models,were built respectively with high fat emulsion on the basis of Letrozole and DHEA（ Dehydroepiandrosterone） models. The models were verified by the smears of vaginal exfoliated cells,the weight growth rate when rats were killed,the lipid levels and the morphological changes of ovarian. Immunohistochemical method was chosen to detect the expression of Bcl-2,Bax in the ovarian follicle granulosa cells of the rat PCOS models. Results： Models were set up successfully. Immunohistochemical results showed that the Bax average grayscale value in the blank controlled group was lower than the Letrozole group,Letrozole improved group,DHEA group and improved DHEA group,while the Bcl-2 average grayscale value in those groups were significantly lower than the blank controlled group,the differences were no statistically significant. Conclusion： The expression of Bcl-2 is down regulated while Bax is up in ovarian follicle granulosa cells,it is inferred that the change of expression is likely to be the key factor of the increased apoptosis in the granulosa cells of the improved PCOS rat model.