超临界CO_2萃取法与水蒸气蒸馏法提取枸骨叶挥发油的GC-MS分析

GC-MS analyze on chemical components of essential oil from Ilex cornuta Lindl. et Paxt. leaves extracted by supercritical CO_2 fluid and steam distillation

采用超临界CO_2流体萃取法(SFE-CO_2)和水蒸气蒸馏法(SD)提取枸骨叶中的挥发油成分,并运用气相色谱-质谱联用法(GC-MS)分离和分析这两种提取物的化学成分,用峰面积归一化法确定各化合物的相对百分含量,比较两种不同提取方法所得挥发油组分的差异。结果表明,从SFE-CO_2法提取的挥发油中,共检出80个成分,鉴定出其中的47个,占挥发油总量的82.32%,含量最高的组分为蒲公英甾醇;从SD法提取的挥发油中,共检出48个成分,鉴定出其中的15个,占挥发油总量的78.91%,含量最高的组分为β-桉叶醇。两种方法提取枸骨叶挥发油的化学组分与含量差别较大,因此可根据生产需要,选用不同方法提取枸骨叶中的有效成分。

The essential oil from Ilex cornuta Lindl. et Paxt. was extracted by supercritical fluid extraction（ SFE-CO2） and steam distillation（ SD）. The constituents were separated and identified from the essential oil by gas chromatography-mass spectrometry（ GC-MS）. The relative percentage of the constituents was calculated by area normalization method. The chemical constituents of essential oil from Ilex cornuta Lindl. et Paxt. by different extraction methods were analysised and compared. The results showed that in the sample extracted by SFE-CO2,eighty components were separated totally and forty-seven constituents were identified,which occupied 82. 32% of essential oils. The highest levels of components was Taraxasterol（ 65. 27%）. For SD,forty-eight components were separated totally and fifteen constituents were identified,which occupied 78. 91% of essential oils. The highest levels of components was β-Eudesmol（ 35. 25%）. The constituents and content of essential oil extracted by the two methods exhibited significant differences. Therefore,the effective components from Ilex cornuta Lindl. et Paxt. were extracted by different methods according to production needs.