骨肉瘤组织中Ezrin蛋白、E-钙粘蛋白的表达及临床意义

Expression of Ezrin protein and E- cadherin in osteosarcoma tissues and their clinical significance

目的观察骨肉瘤组织Ezrin蛋白、E-钙粘蛋白表达,探讨其在骨肉瘤发生、发展中的作用及临床意义。方法采用免疫组化SP法检测58例骨肉瘤、14例骨样骨瘤、11例骨母细胞瘤和9例反应性新生骨组织石蜡标本中Ezrin蛋白、E-钙粘蛋白的表达,分析Ezrin蛋白、E-钙粘蛋白与骨肉瘤临床病理参数的关系,并对骨肉瘤组织Ezrin蛋白与E-钙粘蛋白表达进行相关性分析。结果骨肉瘤组织Ezrin蛋白阳性表达率高于骨样骨瘤、骨母细胞瘤、反应性新生骨组织(P<0.05),E-钙粘蛋白阳性表达率低于骨样骨瘤、骨母细胞瘤、反应性新生骨组织(P<0.05)。骨肉瘤患者有无肺转移、2年内是否死亡之间Ezrin蛋白、E-钙粘蛋白阳性表达率比较,差异均有统计学意义(P<0.05),且二者表达强度呈显著负相关(r=-0.64,P<0.05)。结论 Ezrin蛋白、E-钙粘蛋白在骨肉瘤组织中呈差异表达,其表达水平与患者转移、预后密切相关。

Objective Observe the expression of Ezrin protein and E- cadherin in osteosarcoma tissues,and explore its role in the occurrence and development of osteosarcoma and its clinical significance. Methods Use immunohistochemical SP method to detect the expression of Ezrin protein and E- cadherin in 58 cases of osteosarcoma,14 cases of osteoid osteoma,11 cases of bone tumor and 9 paraffin specimens ofreactive newly formed bone tissues in malignant bone disease,and then analyze the relation between Ezrin protein,E- cadherin and clinical- pathological parameters,furthermore,make a correlational analyses of the expression of Ezrin protein and E- cadherin in osteosarcoma tissues. Results The positive expression rate of Ezrin protein in osteosarcoma tissues was higher than that of osteoid osteoma,osteoblastoma and newly formed bone tissues in malignant bone disease（ P 〈 0. 05）,while the positive expression rate of E- cadherin was lower than that（ P 〈 0. 05）. There was significant difference regarding the positive expression rate of Ezrin protein and E-cadherin in osteosarcoma patients with or without pulmonary metastasis and death within two years（ P 〈 0.05）,and the expression intensity was significantly negative correlation（ r =- 0. 64,P 〈 0. 05）. Conclusion Ezrin protein and E- cadherin are differently expressed in osteosarcoma tissues,and the expression levels of both factors are closely related to metastasis and prognosis of osteosarcoma patients.