神经干细胞移植治疗血管性痴呆大鼠的实验研究

Experimental Research of Neural Stem Cells Transplantation? in Treatment of Rats with Vascular Dementia

目的 研究神经干细胞移植治疗血管性痴呆大鼠的临床效果.方法 选择年龄在12个月左右的60只健康成年Wistar大鼠作为该次实验对象,实验时间为2015年5—10月,大鼠的体重均在300~400 g左右,用于制备血管性痴呆2VO模型,将大鼠随机分成3组,分别为假手术组、对照组及NSCs组,在移植前后对3组大鼠实施Morris水迷测试.结果 移植后5周、10周时,NSCs组大鼠逃避潜伏期（36.85±7.14）、（40.26±8.11）相对于对照组（52.11±6.57）、（54.10±7.16）有了显著的减少,差异有统计学意义（P〈0.05）.结论 针对VaD大鼠模型采用神经干细胞移植的效果显著,能有效改善大鼠学习记忆能力.

Objective To research the clinical effect of neural stem cells transplantation in treatment of rats with vascular dementia. Methods 60 healthy adult Wistar rats whose weight were between 300 ~400 g and whose ages were about 12 months were selected as the experimental object, and the experimental time was from May 2015 to October 2015, and they were used to prepare vascular dementia 2VO models and randomly divided into the sham-operated group, control group and NSCs group, and the three groups were tested by Morris water maze task before and after transplantation. Results The escape latent periods of rats in the NSCs group obviously decreased at the 5 weeks and 10 weeks after transplantation compared with those in the control group with statistical significance [（36.85±7.14）,（40.26±8.11） vs（52.11±6.57）,（54.10±7.16）]（P〈0.05）. Conclusion The effect of neural stem cells transplantation for Va D rat models is prominent, which can effectively improve the learning and memory abilities of rats.