光化学衍生-高效液相色谱法检测食品中的黄曲霉毒素

Determination of aflatoxins in food by high performance liquid chromatography coupled with post-column photochemical derivatization

目的建立食品中黄曲霉毒素B1、B2、G1、G2的光化学衍生高效液相色谱荧光检测方法。方法用乙腈-水提取样品中的黄曲霉毒素,经多功能柱净化,以38/62的甲醇-水为流动相等度洗脱,高效液相色谱分离,光化学衍生器衍生,荧光检测器检测。结果 4种黄曲霉毒素在24 min内得到良好的基线分离,黄曲霉毒素B1、G1的检出限为0.2μg/kg,黄曲霉毒素B2、G2的检出限为0.1μg/kg。在1.0~5.0μg/kg添加范围内,加标回收率为84.0%~94.6%,相对标准偏差为3.4%~6.5%。结论该方法灵敏度高,操作简便,准确可靠,重现性好,适于对食品中黄曲霉毒素的日常检测。

Objective To develop a method for the determination of aflatoxins B1,B2,G1,and G2 by high performance liquid chromatography（ HPLC） coupled with post-column photochemical derivatization. Methods The aflatoxins were extracted by acetonitrile-water solution,and purified by multifunctional clean-up columns. The extract was separated by HPLC with methanolwater isocratic elution,and quantified by fluorescence detection after photochemical derivatization. Results The baseline separation of aflatoxins B1,B2,G1 and G2 was achieved within 24 minutes. The detection limits of aflatoxins B1,G1 and aflatoxins B2,G2 were 0.2 μg / kg and 0. 1 μg / kg,respectively. The average recovery of standard addition for spiked samples in 1. 0- 5. 0μg / kg was 84.0%-94.6%,and the relative standard deviation was 3.4%-6.5%. Conclusions This method possesses several advantages,including high sensitivity,simplicity,precision and repeatability,and it is applicable to the batch analysis of aflatoxins.