HPLC测定不同干燥方法亳菊中5种成分的含量

Determination of Five Components in Dendranthema morifolium Using Different Dry Methods by HPLC

通过高效液相色谱法建立测定不同干燥方法亳菊中绿原酸、3,5-O-二咖啡酰基奎宁酸、木犀草素、木犀草苷和金合欢素的含量的方法。采用菲罗门C18色谱柱(4.6 mm×250 mm,5μm),乙腈-0.1%磷酸水为流动相,梯度洗脱,流速:1.0m L·min^(-1),检测波长325 nm,柱温30℃。绿原酸41.50~0.83×103μg·m L^(-1)木犀草苷2.45~122.50μg·m L^(-1)、3,5-O-二咖啡酰基奎宁酸15.78~1.58×103μg·m L^(-1)、木犀草素327.33~1.96×103μg·m L^(-1)、金合欢素7.62~762μg·m L^(-1)浓度范围内呈良好线性关系,回归方程分别为y=3.3257x-10.6227(r=1.0000)、y=0.9865x+0.1356(r=1.0000)、y=3.6681x-27.6275(r=0.9999)、y=1.2538x-4.3563(r=1.0000)和y=3.6216x-1.2515(r=1.0000)。加样回收率分别为97.97%、97.49%、97.79%、98.27%、98.90%,RSD均小于5%。该方法快速,简便,重复性好,适合于同时测定亳菊中绿原酸、3,5-O-二咖啡酰基奎宁酸、木犀草素、木犀草苷和金合欢素的含量。

A sensitive and specific high performance liquid chromatography（ HPLC） method was developed to determine chlorogenic acid,3,5- Dicaffeoyl quinic acid,luteolin,luteolin- 7- O- β- D- glucoside and acacetin in Dendranthema morifolium. The sample was extracted by reflux extraction. The chromatographic separation was carried out on a Phenomenex C18column（4. 6 mm × 250 mm,5 μm） with acetonitrile and 0. 1% phosphoric acid as mobile phases at a flow rate of 1. 0 m L·min^-1. The UV detection wavelength was set at 325 nm,and the column temperature was set at 30 ℃. The linear correlation equations for chlorogenic acid,3,5- dicaffeoyl quinic acid,luteolin,luteolin- 7- O- β- D- glucoside and acacetin were y = 3. 3257x- 10. 6227（ r = 1. 0000）,y = 0. 9865 x ＋ 0. 1356（ r = 1. 0000）,y =3. 6681x- 27. 6275（ r = 0. 9999）,y = 1. 2538x- 4. 3563（ r = 1. 0000） 和 y = 3. 6216x- 1. 2515（ r = 1. 0000）,respectively. A good linearity was obtained over the range of 41. 50 ～ 0. 83 × 10^3μg·m L^-1,2. 45 ～ 122. 50 μg·m L^-1,15. 78 ～ 1. 58 × 10^3μg·m L^-1,327. 33 ～ 1. 96 × 10^3μg·m L^-1,7. 62 ～ 762 μg·m L^-1,respectively. The recoveries of were 97. 97%,97. 49%,97. 79%,98. 27%,and 98. 90%,respectively. RSD both were less than 5%. The developed HPLC method was quick,simple and reproducible for the simultaneous determination of Chlorogenic acid,3,5- dicaffeoyl quinic acid,luteolin,luteolin- 7- O- β- D- glucoside and acacetin in Dendranthema morifolium.