主成分分析结合均匀设计法用于优选桂芍巴布贴制备工艺的研究

Optimization of preparation process of Gui-Shao Cataplasm based on principal component analysis combined with uniform design

目的运用主成分分析建立一种中药多指标的综合评价方法,并将其应用于桂芍巴布贴制备工艺的优选研究。方法以巴布剂的外观性状、初黏力、持黏力、剥离强度为指标,应用主成分分析求得总因子得分(F),以F为综合指标,采用均匀设计法对聚丙烯酸钠NP-700、甘羟铝、甘油等的用量进行优化,确定最佳处方比例。结果桂芍巴布贴的最佳处方为NP-700-PVP K-30-甘羟铝-EDTA-2Na-酒石酸-甘油-浸膏(8∶1∶0.25∶0.07∶0.15∶25∶20),酒石酸、PVP K-30、NP-700、甘羟铝、甘油、EDTA-2Na是最主要的影响因素。结论优选的桂芍巴布贴易涂布、膏体均匀、黏性较好,可为桂芍巴布贴的新剂型开发提供基础;同时主成分分析法为中药多指标评价研究提供借鉴。

Objective To establish a comprehensive evaluation method based on principal component analysis（PCA） for the study of multiple indexes of Chinese medicine and to choose the best preparation technology of Gui-Shao Cataplasm with this method. Methods The appearance description, initial bonding strength, endurance bonding strength, and peel strength were taken as indexes, the total factor scores were calculated using PCA and were employed to evaluate the quality of Gui-Shao Cataplasm, the uniform design test was used to optimize the amounts of sodium polyacrylate NP-700, aluminium glycinate, glycerin, etc. for Gui-Shao Cataplasm. Results The optimal ratio of the prescription was as following： sodium polyacrylate NP-700-PVP K-30-aluminium glycinate-EDTA- 2Na-tartaric acid-glycerin-extract（8∶1∶0.25∶0.07∶0.15∶25∶20）. The foremost factors were tartaric acid, PVP K-30, sodium polyacrylate NP-700, aluminium glycinate, glycerin, and EDTA-2Na. Conclusion The optimal prescription has easy ductility, uniform paste, and moderate adhesion, and it could provide the foundation for the development of new prescription of Gui-Shao Cataplasm; The PCA could be a reference for the Chinese medicine research of multiple indexes.