摘要
目的:研究不同产地灯盏细辛药材中野黄芩苷含量。方法:采用高效液相色谱法,色谱柱:十八烷基硅烷键合硅胶柱Diamonsil C_(18)(250×4.6mm),流动相:乙腈-水-磷酸(18∶82∶0.2),流速:1m L.min^(-1),检测波长:282 nm,柱温:40℃。结果:在选定色谱条件下,野黄芩苷分离度良好,线性范围为0.1812μg^2.8997μg,不同产地灯盏细辛药材中野黄芩苷含量差异较大,云南双柏县药材野黄芩苷含量最高。结论:不同产地灯盏细辛含量差异大。
Objective： To determine the content of scutellarin in Dengzhanxixin from different habitats. Method： HPLC was adopted to determine the content of scutellarin. The mobile phase was as following： acetonitrile： water： phosphoric acid （18：82：0.2 V/ V）. The flow rate was 1 mL. min^-1 at 40 ℃. The UV detection wavelength was 282nm. Result： Under the selected chromatography condition, the scutellarin was observed in a good separation and was good linear in the range of 0.1812 μg -2.8997 μg. The content of scutellarin in Dengzhanxixin from different habitats was different. And the content of scutellarin was highest in Dengzhanxixin from Shuangbai County, Yunnan Province. Conclusion： The content is different of Dengzhanxixin from differentthe habitats.
出处
《中药与临床》
2016年第2期18-19,共2页
Pharmacy and Clinics of Chinese Materia Medica
