摘要
目的：建立补肾活血方中人参总皂苷含量测定方法。方法：以人参皂苷Re为对照品,香草醛-高氯酸显色,采用分光光度法于544 nm处测定补肾活血方中人参总皂苷的含量。结果：在50.1-150.3μg范围内,对照品Re的吸光度与含量线性关系良好（r=0.9996）,平均回收率为99.95%,RSD=2.33%（n=6）。结论：该法精密度、重现性良好,可作为补肾活血方人参总皂苷的含量测定方法。
Objective： To establish a method for the content determination of ginsenosides in Bushen Huoxue prescription. Method： With ginsenoside Re as reference substance, the content of ginsenosides in Bushen Huoxue prescription was determined by spectrophotometry after HC104-vanilin staining. Result： The calibration curve of ginsenoside Re was linear in the range of 50.1μg -150.3μg. The average recovery was 99.95% and RSD was 2.33% （n=6）. Conclusion： This method can be used for the quality control of Bushen Huoxue prescription with good precision and reproducibility.
出处
《中药与临床》
2016年第2期39-41,共3页
Pharmacy and Clinics of Chinese Materia Medica
基金
国家自然科学基金项目(81072845
81473735)
关键词
补肾活血方
人参总皂苷
含量测定
分光光度法
Bushen Huoxue prescription
ginsenosides
content determination
spectrophotometry
