几种微藻饵料对缢蛏稚贝脂质营养组成的影响

Effect of several diets on the composition of fatty acids and sterols in juvenile Sinonovacula constricta

通过用4种缢蛏人工养殖中常用的饵料微藻（角毛藻、球等鞭金藻、威氏海链藻、假微型海链藻）,以单种饵料和混合饵料的形式对缢蛏稚贝进行投喂实验,研究不同的微藻饵料对缢蛏稚贝的脂肪酸和甾醇组成的影响。对喂食饵料后的缢蛏稚贝进行脂肪酸甾醇的GC-MS分析,共检测到16种脂肪酸和7种甾醇,不同的单种饵料及混合饵料的缢蛏投喂组的脂肪酸、甾醇差异很大。C16：2在含有威氏海链藻的单种饵料和混合饵料的投喂组被检测到;金藻混合藻饵料的缢蛏投喂组脂肪酸种类最丰富（13~15种）,PUFA百分含量最高,胆甾醇含量不高但富含菜子甾醇。因此,从微藻饵料对缢蛏稚贝的脂质营养化影响上来说,金藻的混合饵料是最佳饵料。

It is investigated that effects of the four microalgae（ Chaetoceros calcitrans,Isochrysis galbana,Thalassiosira pseudonana and Thalassiosira weissflogii） and their mixture,which commonly used as S. constricta rearing diets,on the composition of fatty acids and sterols in juvenile S. constricta. The fatty acid and sterol composition of juveniles were analyzed by GC-MS. The results showed that different diets have effect on fatty acid and sterol compositions in juveniles,and 16 fatty acids and 7 sterols have been detected. C16： 2 detected in T. weissflogii and its mix. Juveniles fed with I. galbana mix was abundant in the species of fatty acids（ 13- 15） and C28： 1（ Δ5/24Me）（ Brassicasterol）. Besides,the PUFA percentage content of Juveniles fed with I. galbana mix was higher than that of others.Above all,we choose I. galbana mix as optimal diet because they made lipids of S. constricta juveniles more nitrification.