摘要
研究并建立针对大田软海绵酸OA(okadaic acid)快速、有效的酶联免疫吸附试验ELISA(Enzyme-Linked ImmunosorbentAssay)定量检测方法,制备了定量检测试剂盒-生物酶传感器,并研发配套的现场快速检测仪器-仿生电子眼(Bionic e-Eye),实现对OA的快速检测。对标准样品的参数进行测定,该OA试剂盒的检测限为0.19μg/L,检测范围为0.2μg/L^5μg/L;样品回收率为75%~125%,精密度在±15%以内。将Bionic e-Eye的检测结果和酶标仪及高效液相色谱HPLC(high performance liquid chromatog-raphy)所测数据的结果进行了比较,结果表明,Bionic e-Eye的性能与酶标仪的检测结果一致。此外,该仪器可以直接得出检测结果,不需要人工计算处理,适用于现场的快速检测,将是一种新的现场快速定量检测OA的技术手段。
Based on Enzyme-linked immunosorbent assay（ELISA）,test kit,namely,enzyme sensor and the corre-sponding instrument was prepared,Bionic e-Eye for rapid,effective and quantitative detection of okadaic acid（OA）on-site. The detection limit of the kit is 0.19 μg/L and the range is among 0.2 μg/L and 5 μg/L. The recovery rate isamong 75% and 125% and the accuracy is within±15%. The performance of Bionic e-Eye is consistent with micro-plate reader and high performance liquid chromatography（HPLC）. In addition,without artificial calculation,theinstrument can show the results directly on the screen,which is very suitable for rapid detection,and will be a newtechnology for rapid and quantitative detection of OA on site.
出处
《传感技术学报》
CAS
CSCD
北大核心
2016年第5期633-636,共4页
Chinese Journal of Sensors and Actuators
基金
国家海洋公益专项项目(201305010)
教育部博士点基金项目(20120101130011)
国家自然科学基金项目(31571004)
