黄芪甲苷对皮质酮损伤海马神经细胞的保护作用及GCR和SYN1表达的影响

Effect of astragaloside on the activity and expression of GCR and SYN1 of corticosterone injured hippocampal neurons

目的:观察黄芪甲苷对高浓度皮质酮损伤海马神经细胞活性及学习记忆相关蛋白GCR及SYN1表达的影响。方法:采用CCK8法,FDA/PI双荧光染色观察皮质酮对原代培养新生24h大鼠海马神经细胞存活率的影响,同时观察黄芪甲苷的保护作用;利用实时定量PCR及Western blot法检测黄芪甲苷对皮质酮损伤细胞GCR和SYN1 mRNA及蛋白表达的影响。结果:与对照组比,皮质酮能够时间剂量依赖性的降低神经细胞存活率,而0.1μmol/L、1μmol/L及10μmol/L黄芪甲苷及10μmol/L美金刚均能不同程度地提高损伤细胞的存活率;1μmol/L黄芪甲苷能上调损伤细胞GCR和SYN1 mRNA及蛋白表达。结论:黄芪甲苷对高浓度皮质酮损伤神经细胞具有保护作用,并能够上调学习记忆相关蛋白GCR和SYN1的表达。

Objective： To observe the effect of astragaloside on the activity and the expression of learning and memory related genes GCR and SYN1 of corticosterone injured hippocampal neurons. Methods： CCK8 method and FDA / PI double fluorescent staining were used to observe the survival rate of corticosterone treated hippocampal neurons primarily cultured from newborn SD rat,and to assess the protective effect of astragaloside. Real-Time PCR and Western blot were used to detect the expression of GCR and SYN1 mRNA and protein. Results： Compared with the control,the survival rate of neurons decreased after treatment with corticosterone in a time and dose dependent manner（ P 〈 0.01）; 0. 1μmol / L,1μmol / L and 10μmol / L astragaloside and memantine improved the survival rate of corticosterone damaged neurons（ P 〈0. 01） in varying degrees; 1μmol / L astragaloside increased GCR and SYN1 mRNA and protein expressions of the damaged neurons. Conclusion： Astragaloside could protect neurons from the damage of high concentration corticosterone and adjust the expression of learning and memory related genes GCR and SYN1.