五味子抗氧化活性组分筛选

Screening of Antioxidant Active Components of Schisandra chinensis

目的:筛选五味子抗氧化的活性组分。方法:以1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除能力(半数清除浓度,IC50)为指标,以乙醇体积分数、料液比及提取时间为考察因素,设计正交试验优化五味子的提取工艺并进行验证;采用大孔树脂吸附法对五味子提取物进行分离纯化,以水和10%、30%、50%、70%、95%乙醇梯度洗脱,分别得到组分SC-0、SC-10、SC-30、SC-50、SC-70、SC-95,以IC_(50)和总抗氧化能力(ABTS法测定)为考察指标(以维生素C为阳性对照),优选五味子抗氧化活性组分;采用高效液相色谱法对五味子各组分的5种木脂素含量进行测定。结果:五味子的最优提取工艺为60%乙醇、料液比1∶14、提取2.0 h;验证试验中IC_(50)平均值为23.81 mg/ml(RSD=0.52%,n=3);SC-0无抗氧化活性,其余各组分IC50值由低到高(即清除能力大小)为阳性对照>SC-50>SC-30>SC-95>SC-70>SC-10;总抗氧化能力大小为SC-50>阳性对照>SC-30>SC-70>SC-95>SC-10;各组分中5种木脂素总含量大小为SC-70>SC-50>SC-95>SC-30。结论:50%乙醇洗脱物为五味子抗氧化的活性组分。

OBJECTIVE：To screen antioxidant active components of Schisandra chinensis.METHODS：The orthogonal test was adopted to optimize extraction technology using DPPH free radical scavenging activity（IC50）as index and ethanol volume fraction,material-liquid ratio and extraction time as factors,and the verification test were made.The fractions（SC-0,SC-10,SC-30,SC-50,SC-70,SC-95）were made by extracting and purifying S.chinensis with macroporous resin with water and 10%,30%,50%,70% and 95% ethanol.With IC50 and total antioxidant capacity（determined by ABTS method）as indexes（vitamin C as positive control）,the antioxidant active components of S.chinensis were optimized.The contents of 5 kinds of lignan in different positions of S.chinensis were determined by HPLC.RESULTS：The optimal extraction condition of S.chinensis was as follows as60% ethanol,material-liquid ratio of 1 ∶ 14,extracting for 2.0 h.The average IC50 of DPPH free radical scavenging activity was23.81 mg/ml（RSD=0.52%,n=3）in verification test.SC-0 did not have antioxidant abilities.DPPH free radical scavenging activity of those components（ie.the IC50 value from low to high） were in the following order of positive controlSC-50SC-30SC-95SC-70SC-10;total antioxidant ability of them were in the following order of SC-50positive controlSC-30SC-70SC-95SC-10;the contents of 5 types of lignan in different components were in the following order of SC-70SC-50SC-95SC-30.CONCLUSIONS：The antioxidant active component of S.chinensis is 50% ethanol eluate.