滑石粉烫狗鞭的工艺优化及质量标准研究

Study on the Optimization of Processing Technology of Dog Testes and Penis Processed with Talcum Powder and Its Quality Standard

目的：筛选滑石粉烫狗鞭的最优工艺,并为其质量控制标准制订提供参考。方法：以粉碎率和醇溶性浸出物含量的综合评分为评价指标,采用L_9（3～4）正交试验优化烫制温度、烫制时间和滑石粉用量,并进行验证试验;按2015年版《中国药典》相关方法对制狗鞭中水分、灰分、含氮量及醇溶性浸出物含量进行测定,采用氨基酸自动分析仪测定制狗鞭中氨基酸的含量。结果：最优炮制工艺为100 kg狗鞭加40 kg滑石粉、烫制温度为350～380℃、烫制时间为4 min。验证试验结果表明,3批炮制品的粉碎率和醇溶性浸出物含量平均值分别为80.2%、15.7%（RSD分别为0.95%、2.30%,n=3）。制狗鞭中水分为5.7%、灰分为18.7%、含氮量为8.3%、醇溶性浸出物含量为15.7%;含有16种氨基酸,总量为42.44%。结论：优化的滑石粉烫狗鞭工艺稳定、可行;建议水分、灰分、含氮量、醇溶性浸出物含量及氨基酸含量可作为制狗鞭质量标准的控制指标。

OBJECTIVE：To screen the optimal technology of dog testes and penis processed with talcum powder,and to provide reference for the formulation of quality control standard.METHODS：Taking the comprehensive score of crushing rate and the content of alcohol soluble extract as evaluation indexes,L_9（34）orthogonal test was used to optimize processing temperature,processing time and the amount of talcum powder;validation test was conducted.According to related method in Chinese Pharmacopoeia（2015 edition）,the contents of moisture,ash,nitrogen and alcohol soluble extract were determined,and automatic amino acid analyzer was used to determine the content of amino acids in samples.RESULTS：The optimal processing technology were that100 kg dog testes and penis should add into 40 kg talcum powder;the processing temperature was 350-380 ℃;processing lasted for 4 min.Results of verification test showed that the average crushing rate and the content of alcohol soluble extract were 80.2%（RSD=0.95%,n=3）and 15.7%（RSD=2.30%,n=3）.In processed dog testes and penis,the contents of moisture,ash,nitrogen and alcohol soluble extract were 5.7%,18.7%,8.3%,15.7%,respectively.Besides,there still were 16 kinds of amino acids,and their total content was 42.44%.CONCLUSIONS：The optimized talcum powder processing technology of dog testes and penis is stable and practical.The content of moisture,ash,nitrogen,alcohol soluble extract and amino acid can be used as important quality standard control indexes for processed dog testes and penis.