人连接蛋白43基因启动子活性区域分析

Analysis on Active Reagions of the Promoter of Human Connexin 43 Gene

利用PCR技术从基因组DNA中获得不同长度的Cx43基因启动子片段,克隆至荧光素酶报告基因载体pGL3-Basic中,瞬时转染成纤维细胞,通过荧光素酶活性检测,分析不同启动子区域的转录调控能力。结果显示Cx43基因不同长度的启动子荧光素酶报告基因载体被成功构建,它们在成纤维细胞中的活性不同:535^-1区域可能为Cx43基因启动子的核心转录调控作用区,这为进一步研究Cx43在成纤维细胞中的转录调控特点奠定了基础。

Different length of Cx43 promoter fragment was obtained from genomic DNA by PCR technique.They were cloned to the luciferase reporter gene vector pGL3-Basic and then transiently transfected into fibroblasts.The transcriptional regulation ability of Cx43 different promoter regions was analyzed by luciferase activity assay.The results revealed that Cx43 promoter luciferase reporter vector was successfully constructed,their activity in fibroblasts was different.And the-535^-1 region might be the core transcriptional regulatory region of Cx43 promoter.The activity region of Cx43 gene promoter was determined,which laid a foundation for further study on the transcriptional regulation of Cx43 in fibroblasts.