贵州大眼鳜3个群体rDNA ITS-1序列分析

Sequence Analysis of r DNA ITS-1 from three Populations of Siniperca kneri in Guizhou

运用PCR扩增技术及DNA测序技术,对贵州3个大眼鳜群体的ITS-1序列进行了分析,结果显示3个大眼鳜群体存在序列长度多态性,主要有753 by和755 by两种类型,GC(72.8%)平均含量远高于AT含量(27.3%)洪检测到6个多态位点,产生6种单倍型;3个群体各自都表现出较低的遗传多样性水平,从总体上看,单倍型多样性指数为0.622,表明贵州大眼鳜群体的遗传多样性较丰富。在贵州大眼鳜ITS-1序列中发现(GC)_n、(CT)_n、(CG)_n、(TCG)_n4种微卫星序列,其中(GC)_n出现的频率最高。群体间的遗传分化指数(Fst)和基因交流值(Nm)均表明,铜仁大眼鳜群体与沿河大眼群体遗传分化小,有基因交流而关岭大眼鳜群体与这两个群体间,遗传分化大,缺乏基因交流。

The sequences of internal transcribed spacer 1（ITSl） in the specie were analyzed using the methods of PCR and DNA sequencing according to the 151 individuals of Siniperca kneri in three wild populations from Tongren,Yanhe,Guanling.The length polymorphism was observed in three populations of Siniperca kneri,with two types of 753 bp and 755 bp in the length.The mean contents of GC were significant higher than AT.6polymorphism loci and 6 haplotypes were dected in ITSl.The three populations of Siniperca kneri had low genetic diversity respectively,the total haploidtype diversity was 0.622,suggesting the abundant genetic diversity in three populations of Siniperca kneri.Four kinds of microsatellites were（GC）_n,（CT）_n,（CG）_n,（TCG）_n respectively found in ITSl sequences in the specie,the type of（GC）_n had highest frequency.Fst value and Nm value among the three populations of Siniperca kneri suggested that between Tongren and Yanhe Population have weak genetic differentiation,the Guanling population lacked of gene exchange to these two populations.