十字花科黑腐病菌dsbA基因控制的亚细胞蛋白质组分析

Identification of Subcellular Proteomes Controlled by dsbA in Xanthomonas campestris pv. campestris

本研究以十字花科黑腐病菌(Xarcthomorcas campestris pv.campestris,Xcc)8004菌株为研究对象,采用双向电泳—质谱技术分析比较了dsbA1A2突变体和野生型菌株的分泌蛋白、周质蛋白表达谱。与野生型菌株相比,选取的31个差异蛋白中,14个蛋白点显著上调,17个蛋白点显著下调。对这些蛋白质点进行质谱鉴定和分析,结果显示,dsbA1A2基因的缺失导致了周质蛋白中与β桶结构膜蛋白形成有关的伴侣蛋白SurA的含量降低,几种未知功能的分泌蛋白的分泌量减少以及外膜蛋白OmpW、OmpW1在周质空间的积累。推测这些蛋白质对Xcc 8004的致病性至关重要,为进一步研究Xcc 8004中DSB系统的作用机理提供了依据。

This study analyzed the expression spectrum of the secreted proteins and periplasmic proteins between the dsbA1A2 mutant and the wild type strain in cruciferous black rot pathogen（Xanthomonas campestris pv.campestris,Xcc） 8004 strains.Two-dimensional electrophoresis combined with mass spectrometry identification method were utilized.Compared with the wild-type strains,dsbA1A2 mutant showed 14 significantly up-regulated and 17 significantly down-regulated proteins in the 31 selected proteins.Identification and analysis of these proteins by mass spectrometry,showed that the knockout of dsbA1A2 leaded to the chaperone SurA,which was associated with the formation of beta-barrel membrane proteins,decreased in periplasmic proteins.The dsbA1A2 mutant strain exhibited the reduction of several kinds of unknown function proteins,and also caused accumulation of the outer membrane protein OmpW and OmpW1 in the periplasmic space.It speculated that these proteins were critical to the pathogenicity of Xcc 8004.Therefore,this study might provide the theory basis accounting for the further mechanism research of DSB system in Xcc 8004.