利用黄瓜加密图谱定位花相关性状QTLs

Mapping of QTLs Associated with Flower-related Traits Using the Encryption Map in Cucumber(Cucumis sativus L.)

本研究利用强雄的华北类型品系S94和全雌的欧洲类型品系S06构建的224个F_(6:7)代重组自交系(RIL)群体对不同季节(F_7/秋,F_7/春两季)黄瓜花相关性状(始花节位ffn,始雌花节位fffn和雌花率sex)劝表型进行研究。利用构建的加密图谱采用WinQTLcart 2.5软件检测3个花相关性状QTL位点。结果显示:(1)构建包含7个连锁群,610个标记的加密图谱覆盖基因组749.2 cM,平均标记间距为1.2 cM;(2)3个花相关性状在F_7/秋与F_7/春两季共检测15个QTLs位点(F_7/秋12个,F_7/春9个)分布于除第4染色体外的黄瓜染色体上。在花相关性状中,春秋两季相同性状都有稳定检测的QTL有6个,其中3个QTLs雌花率sex6.2(18.2%,秋和37.7%,春)、始花节位ffn6.1(16.7%,秋和12.0%,春)和始雌花节位fffn6.1(18.4%,秋和40.1%,春)在两季的贡献率都高于10%,仅1个位点始花节位ffn1.3在单一季节表现高的贡献率(>10%)。

A set of 224 F6：7 recombinant inbred lines（RILs） derived from a cross between S94（Northern China type with the monoecious） and S06（Northern European type with the gynoecious） was used to investigate the phenotypic data of three flower-related traits（first flower node（ffn）,first female flower node（fffn）,and female flower ratio（sex） in different seasons（F7 in spring and F7 in autumn）.The encryption map and WinQTLcart 2.5 software were used to identify the quantitative trait loci for three flower-related traits.Result showed that（1） an encryption map including 610 markers was grouped into seven linkage groups and spanned 749.2 cM with an average marker density of 1.2 cM.（2） Using the F6：7 derived families,a total of 15 QTLs for flower-related traits were detected in F7/autumn（12） and F7/spring（9） and distributed in all chromosomes except for Chromosome 4^th.Of all flowerrelated traits,6 QTL loci for the same traits were detected both in spring and autumn,of which female flower ratio sex6.2（18.2%,autumn,37.7%,spring）,first flower node ffn6.1（16.7%,autumn,12.0%,spring） and first female flower node fffn6.1（18.4%,autumn,40.1%,spring） had stable position and higher contribution in both seasons,with a loci（first flower node ffn1.3） showing high contribution（〉10%） in a single season.