低分子肝素对高脂血症性胰腺炎大鼠VEGF表达的影响

Effect of Low Molecular Weight Heparin on the Expression of VEGF in Rats with Hyperlipidemic Pancreatitis

目的研究低分子肝素对大鼠高脂血症性胰腺炎胰腺微循环的作用机制。方法将72只健康SD大鼠随机分成假手术组、模型组和治疗组各24只,每组再随机分成四个亚组。先采用高脂饮食建立高脂血症模型,模型组和治疗组制作大鼠急性胰腺炎模型,制模成功后治疗组分别于0、6、12、18 h开始使用低分子肝素皮下注射,各亚组均间隔6 h重复皮下注射一次,每亚组均干预4次;模型组和假手术组同时间点皮下注射等量生理盐水对照。结果大鼠胰腺血流速度模型组低于假手术组,治疗组高于模型组(P<0.05);胰腺组织病理学评分模型组高于假手术组,治疗组低于模型组(P<0.05);模型组各亚组胰腺血管内皮生长因子(VEGF)蛋白表达、VEGF m RNA表达水平均高于假手术组,而治疗组均低于模型组(P<0.05)。结论大鼠高脂血症性胰腺炎,VEGF与微循环障碍密切相关;低分子肝素可能通过下调胰腺组织VEGF水平来改善胰腺微循环障碍,减轻胰腺组织进一步损害,且早期干预价值更大。

Objective To study the action mechanism of low molecular weight heparin on pancreatic microcirculation of rats with hyperlipidemic pancreatitis. Methods 72 healthy SD rats were randomly divided into sham operation group, model group and treatment group with 24 rats in each group. Then each group was randomly divided into four subgroups. The rats were first used the high fat diet to establish hyper]ipidemia model, the model group and treatment group was to make acute pancreatitis rat models, after the model success, the treatment group were given low molecular heparin subcutaneous injection at 0, 6, 12 and 18 h, the subgroups were repeated the rejection per 6 hours for four times per; model group and sham operation group were injected with equivalent saline at the same time. Results The pancreatic blood flow velocity in model group was lower than that in the sham operation group, the treatment group was higher than that in the model group （P〈0.05）. The pathological score in the model group was higher than that in sham operation group, the treatment group was lower than that in model group （P〈0.05）. The expression of VEGF protein and mRNA VEGF expression in the model group were significantly higher than those in the sham operation group, the treament group was lower than that in the model group （P〈0.05）. Conclusion VEGF is closely related to the disturbance of mierocirculation in rats with hyperlipidemia. The low molecular weight heparin may down regulate the VEGF level of pancreatic tissue to improve the pancreatic microcirculation disturbance, and reduce the damage of pancreatic tissue, and the value of early intervention is greater.