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转基因抗除草剂玉米DAS-40278-9品系特异实时荧光定量PCR检测方法的建立 被引量:5

Establishment of Event-specific Quantitative PCR of Event DAS-40278-9 Herbicide-tolerant Maize
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摘要 根据转基因抗除草剂玉米DAS-40278-9外源基因的旁侧序列,采用ABI Primer Express 3.0设计引物和Taq Man探针,建立转基因抗除草剂玉米DAS-40278-9品系特异实时荧光定量PCR检测方法。采用该检测方法对DAS-40278-9玉米含量为1%(质量分数)的标准样品进行检测,结果显示,采用建立的方法获得的标准曲线,通过斜率、相关系数、扩增效率判断是可靠的,待测样品的定量检测结果为1.024%,非常接近真实值。本试验表明建立的转基因抗除草剂玉米DAS-40278-9品系特异定量PCR检测方法的特异性好,灵敏度和准确度高,值得推广应用。 Event-specific quantitative PCR detection method of genetically modified maize(Event DAS-40278-9)was established. PCR primers and Taqman probe were designed according to the flanking sequence of exogenous gene of Event DAS-40278-9 by the ABI Primer Express 3.0. Sample containing 1% of Event DAS-40278-9 was tested. The results showed that the standard curve obtained was credible through the slope, the correlation coefficient, amplification efficiency. The detection result of sample was 1.04%, closed to the true content. It was proved that the specificity, sensitivity and precision of this method was relatively high and could be used to test GMO samples.
出处 《中国食品学报》 EI CAS CSCD 北大核心 2016年第5期147-154,共8页 Journal of Chinese Institute Of Food Science and Technology
基金 四川省质监局标准化技术体系研究项目(ZYBZ2013-39) 四川省财政现代农业技术创新与示范专项(2014CXSF-040) 四川省教育厅自然科学一般项目(15ZB0331)
关键词 DAS-40278-9 z SSIIb 旁侧序列 定量PCR 检测 event DAS-40278-9 z SSIIb flanking sequence quantitative PCR detection
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参考文献24

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