多种烟杆降解菌株的筛选及其协同作用比例优化

Screening of Several Varieties of Tobacco Rod Degradation Strains and Ratio Optimization of Synergistic Effect

为了对废弃烟杆再利用,自行筛选到几种能够利用废弃烟杆进行高温好氧堆肥的高效微生物菌株,对分解出的几种菌株进行初步检测并对菌株协同作用的组合和配比做了进一步试验。结果表明,筛选获得的3组降解烟杆纤维素的菌株均能在以烟杆为唯一营养源的培养基上生长,且具有较强降解纤维素的能力,菌株编号分别为H2568、B1、M1。对这3种菌株的协同作用试验显示,H2568、B1、M1以1.0∶1.0∶1.5的生物量比例组合时具有最强的降解烟杆的能力,其纤维素降解率、半纤维素降解率、木质素降解率分别达到了56%、70%、33%,最高纤维素酶活力达到625.44 U/m L。

In order to reuse the waste tobacco stalk,several microbial strains can use waste tobacco rod high-temperature aerobic composting were screened,preliminary detection of several decomposed strains was conducted,and the further testing for strains synergistic effect of the combination and proportion was carried out.The results showed that three groups of degradation of tobacco stalk cellulose strains can grow in the culture medium which with tobacco stems as the sole source of nutrition, and has strong cellulose degradation ability, number of strains were H2568, B 1, M 1. Synergistic test indicated when biomass ratios of H2568 ,B 1, M1 with 1.0：1.0：1.5,combination with the strongest degradation of the cigarette rod ability, the degradation rate of cellulose, hemicellulose degradation rate ,lignin degradation rate reached 56%, 70%, 33%, the highest fiber prime enzyme activity reached 625.44 U/mL.