鱼油中EPA与DHA不同配比对降血脂作用效果的比较

Improving Effects of Hyperlipidemia of Different Contents of EPA and DHA in Fish Oil in Rats

目的通过高脂血症大鼠模型研究鱼油中二十碳五烯酸(EPA)与二十二碳六烯酸(DHA)的不同配比在降血脂方面的变化。方法给予70只雄性SD大鼠采用高脂高糖乳剂灌胃10 d,按总胆固醇(TC)水平随机分为模型对照组、非诺贝特胶囊组(28 mg/kg)、海豹油组(333 mg/kg)、鱼油A(167 mg/kg)、鱼油C(150 mg/kg)、鱼油D(183 mg/kg)、鱼油E(167 mg/kg),10只/组,每天给予高脂高糖乳脂及治疗,连续30d。治疗结束后,检测各组动物大鼠血清(TC)、甘油三酯(TG)、高密度脂蛋白(HDLC)、低密度脂蛋白(LDLC)、白介素-2(IL-2)、白介素-6(IL-6)及肝脏系数,并观察肝脏组织病理改变。结果与模型对照组相比,0.028 g/kg非诺贝特胶囊可以降低高脂血症大鼠的TC、TG、HDLC、LDLC含量,减轻肝细胞脂肪变性程度,但可增大肝脏系数(P<0.05或P<0.01);海豹油可以降低TC、TG、HDLC、LDLC含量(P<0.01),鱼油A、鱼油E可以降低TC、HDLC、LDLC含量(P<0.01),鱼油D可以降低TC、TG、HDLC、LDLC含量(P<0.01),但海豹油、鱼油A、鱼油C、鱼油D、鱼油E均未见减轻肝细胞脂肪变性程度(P>0.05)。结论对于高脂血症大鼠模型,鱼油A、鱼油E均具有降低TC、HDLC和LDLC的作用,鱼油D具有降低TC、TG、HDLC、LDLC的作用,鱼油C不具有降低TC、TG、HDLC、LDLC的作用。

Objective To observe the therapeutic effect of different contents of EPA and DHA in fish oil on Hyperlipidemia of rats. Method 70 male SD rats were fed with high-fat diet for 10 days,then randomly divided into seven groups according to TC levels： disease model control group,Fenofibrate group（ 28 mg / kg）,Seal Oil group（ 333 mg / kg）,fish oil A group（ 167 mg / kg）,fish oil C group（ 150 mg / kg）,fish oil D group（ 183 mg /kg）,fish oil E group（ 167 mg / kg）,ten rats in each group,respectively. Rats were treated with high-fat diet and drugs for another 30 days. Then the levels of TC,TG,HDLC,LDLC,IL-2,IL-6,the ratio of liver weight and body weight,and histopathology changes of the liver were examined. Result Compared to the disease model group,Fenofibrate can decrease the level of TC, TG, HDLC, LDLC, the degree of hepatic steatosis of Hyperlipidemia of rats,but increase the ratio of liver weight and body weight（ P 〈0. 05 or P 〈0. 01）; Seal Oil decreased the level of TC,TG,HDLC,LDLC（ P 〈0. 01）; fish oil A and fish oil E decreased the level of TC,HDLC,LDLC（ P 〈0. 01）; fish oil D decreased the level of TC,TG,HDLC,LDLC（ P 〈0. 01）; fish oil C did not decrease the level of TC,TG,HDLC,LDLC（ P 〉0. 05）; all of them did not decrease the degree of hepatic steatosis of Hyperlipidemia of rats. Conclusion Fish oil A,fish oil D and fish oil E can improve Hyperlipidemia of rats model to a certain extent.