摘要
研究建立了鸡血浆中替米考星的高效液相色谱（HPLC）检测方法。血浆中替米考星采用甲醇和三氯甲烷提取，流动相为乙腈：四氢呋喃：二丁胺磷酸缓冲液：超纯水（110：55：25：810，v／v），紫外检测波长286nm，流速1mL／min。结果显示，替米考星标准工作液在O．05。10mg／L范围内呈良好线性关系（R^2≥0．9998）。血浆中替米考星添加浓度为5．0、0．5、0．05mg／L时，回收率为97．0％～99．8％；日内和日间变异系数分别小于7．2％和7．7％。该方法前处理简单省时，不用固相萃取小柱净化，节约试验成本，为替米考星的检测提供了方法学基础。
This study established a high performance liquid chromatography （HPLC） method for determination of tilmicosin in plasma. Methanol and chloroform were used for extraction of tilmicosin from plasma. Mobile phase was as following： acetonitrile,tetrahydrofuran,butyl amine phosphate buffer,uhrapure water with the proportion of 110：55：25：810 （v/v）. UV detection with wavelength of 286 nm was used for detector, the flow rate was 1 mL/min. Tilmicosin standard working solution within the scope of 0.05 to 10 mg/L showed good linear relationship （R2≥0.9998）. The recovery rate was 97.0% to 99.8% at concentration of 5.0,0.5,0.05 mg/L. The intra-day and inter-day coefficient of variation were less than 7.2% and 7.7% respectively. Pre-treatment of the method was simple, timesaving and cost-effective without use of solid phase extraction column,which provided methodology basis for tilmicosin determination.
出处
《中国家禽》
北大核心
2016年第12期21-24,共4页
China Poultry
