高表达Bcl-2下调喉癌细胞黏附能力的分子机制探讨

The investigation of molecular mechanism of the high expression of Bcl-2 down-regulating cell adhesion in laryngeal carcinoma cells

目的:探讨高表达的Bcl-2对喉癌细胞黏附能力的影响。方法:建立并鉴定高表达Bcl-2的稳定细胞株,采用黏附实验检测细胞的黏附能力,流式细胞仪和Western blotting实验分析细胞表面integrin表达的变化。结果:成功构建高表达Bcl-2细胞株,下调了细胞的黏附能力,并降低了细胞表面integr inα2β1的表达,而未见对integr inαv、integr inβ4、integr inα3和integr inα5的表达的影响。结论:高表达Bcl-2可能通过下调了细胞表面integrinα2β1的表达从而减低细胞的黏附能力。

Objective： To investigate the effect of high expression of Bcl-2 on the cell adhesion ability. Methods： The stable cells of high expression of Bcl-2 were built and identified. The adhesion ability was assessed by adhesion assay. The expression of integrin was analyzed by FLOW and Western blotting. Results： Bcl-2 stable cells were successfully built. Bcl-2 overexpression decreased the cell adhesion and the expression of integrin α2β1, however, it did not affect the expression of integrin αv, integrin β4, integrin α3 and integrin α5. Conclusion： High expression of Bcl-2 may reduce the cell adhesion by down-regulating the expression of integrin α2β1.