大豆异黄酮对去卵巢大鼠脾脏NGF、IL-2蛋白表达的影响

Effect of soybean isoflavone on NGF,IL-2 protein in ovariectomized rat's spleen

为了探讨大豆异黄酮(SI)对去卵巢大鼠脾脏中神经生长因子(NGF)、白细胞介素2(IL-2)蛋白表达的影响,本研究在成功建立去卵巢大鼠模型的基础上,对去卵巢大鼠分别隔天皮下注射高(1.5 mg·kg^-1)、中(1.0 mg·kg^-1)、低(0.5 mg·kg^-1)剂量的SI,假手术组(仅手术而不去卵巢)和溶媒组(去卵巢)大鼠隔天皮下注射溶媒剂(乙醇)。补充SI至14 d和42 d时,每组随机剖杀5只大鼠,采用免疫组织化学方法,对大鼠脾脏NGF、IL-2蛋白的表达变化进行研究。结果表明,脾脏中广泛分布着NGF、IL-2蛋白,主要分布于被膜下方的红髓区,在白髓分布量极少。去卵巢后,大鼠脾脏中NGF、IL-2蛋白的表达强度和阳性细胞数目均显著下降,而且随着去除卵巢时间的延长下降加剧。体外补充SI后14 d,各剂量组和42 d低、中剂量组仅有部分恢复,变化不显著;补充高剂量SI 42 d后,脾脏NGF、IL-2蛋白的表达强度和阳性细胞数目基本恢复至假手术组的水平。大豆异黄酮能上调NGF、IL-2蛋白在脾脏内的表达,并呈现时间和剂量的依赖性。

To study the effect of soybean isoflavone on NGF , IL-2 protein in the ovariectomized rat＇s spleen, young female SD rats were ovariectomized successfully , and then were supplied with soy isoflavone （ SI ） in high dosage （1.5 mg· kg^-1）, medium dosage （1.0 mg· kg^-1）, low dosage （0.5 mg· kg^-1）, respectively.The rats of sham op-eration group and the control group were supplied with solvent agent .All of the above groups endured hypodermic in-jection once every other day .After 14 and 42 d treatment, 5 rats per group were killed for study .The expression and distribution of NGF, IL-2 in spleen were studied by immunohistochemical methods .The NGF, IL-2 protein were mainly distributed in red pulp close to the capsule .The intensity and number of NGF , IL-2 positive cells of the ovariectomized rats were significantly decreased , and after ovariectomizing , the damage was enhanced with prolonged time.After soy isoflavone treatment , the intensity and number of NGF and IL-2 positive cells of ovariectomized rats increased and showed a time-dependent manner .During the same period , with the increased concentration of soy isoflavone , the intensity and number of NGF and IL-2 positive cells increased and showed a dose-dependent manner . The above results suggested that soy isoflavone could up-regulate the expression of NGF and IL-2 in a time-and dose-dependent manner .