摘要
将草鱼分为对照组和处理组,对照组试验期内置于正常养殖用水中饲养,处理组先暴露在Hg^(2+)浓度为0.5 mg·L-1水体中,24 h后置于正常养殖用水中进行恢复饲养。各组分别于恢复饲养0(即Hg^(2+)暴露24h)、5、12、21 d取样,测定鳃、肝胰脏、脾脏和肾脏组织中超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶(glutathion and peroxidase,GPx)活性,以及还原型谷胱甘肽(glutathione,GSH)和丙二醛(malondialdehyde,MDA)的含量。结果显示,与对照组相比,处理组鳃SOD、GPx活性(0、5 d)和CAT活性(0、5、12 d),以及GSH(5 d)、MDA(5、12、21 d)含量显著上升(P<0.05),GPx活性(12、21 d)和GSH、MDA含量(0 d)显著下降(P<0.05,P<0.01);肝胰脏SOD、CAT、GPx活性(0 d)和GSH(21d)、MDA(0、5 d)含量显著上升(P<0.05),CAT(5、12 d)活性、GSH(0、5、12 d)和MDA(12、21 d)含量显著下降(P<0.05)。处理组脾脏SOD、CAT、GPx活性在试验期内与对照组相比无显著变化(P>0.05),GSH含量(0 d)极显著下降(P<0.01),GSH(12 d)和MDA(0 d)含量显著上升(P<0.05);肾脏SOD(0、5、12 d)、GPx(0 d)活性和GSH(12 d)、MDA(0 d)含量显著上升(P<0.05),CAT(0、5、12 d)活性和GSH(0、5 d)含量显著下降(P<0.05)。试验期内,处理组各组织SOD、CAT活性均恢复到对照组水平,肝胰脏GSH含量得到反弹性恢复,MDA含量降到了较对照组更低的水平,鳃GPx活性及MDA含量一直未恢复至对照组水平。
This study was aimed to investigate the effect of Hg 2+on oxidative damage and antioxidant capacity of grass carp ( Ctenopharyngodon idellus) .The experimental fish were divided into control and treatment groups .The control group fish was raised in cultivated water , while the treatment group fish was exposed to 0.5 mg· L^-1 Hg^2+for 24 hours first, and then transferred in cultivated water .Afterwards, they were sampled at 0 (24 h Hg^2+exposure), 5, 12, 21 d to test the superoxide dismutase (SOD), catalase (CAT), glutathion and peroxidase (GPx) activities as well as glutathione ( GSH) and malondialdehyde ( MDA) contents in their gill , hepatopancreas , spleen and kid-ney.It was shown that compared with the control group , the SOD, GPx activities (0, 5 d), CAT activity (0, 5,12 d) and GSH (5 d), MDA (5, 12, 21 d) contents in gill in treatment group increased significantly (P〈0.05). In contrast, GPx activity (12, 21 d), GSH and MDA contents (0 d) decreased significantly(P〈0.05).The SOD, CAT, GPx activities(0 d) and GSH (21 d),MDA (0,5 d) contents in hepatopancreas in treatment group rose sharply (P〈0.05).However, CAT activity(5, 12 d), GSH (0, 5, 12 d) and MDA (12, 21 d)contents dropped significantly ( P〈0.05 ,P〈0.01 ) .As for spleen , there was no significant difference on SOD , CAT and GPx activi-ties between control and treatment group during the experimental period (P〉0.05).While, the GSH content (0 d) was lowered significantly(P〈0.01),and the GSH (12 d) and MDA(0 d) contents were elevated remarkably (P〈0.05).The SOD (0, 5,12 d), GPx(0 d) activities and GSH (12 d), MDA (0 d) contents in kidney in treatment group were increased significantly (P〈0.05).Nevertheless,CAT (0,5,12 d) activity and GSH(0,5 d) content were decreased remarkably ( P〈0.05 ) .This test indicated that for grass carp , the SOD and CAT activities of organ tissues were recovered to the normal level .In hepatopancreas , GSH content exceeded the control group level ( P〈0.01 ) , and MDA content was significantly lower than that of control group ( P〈0.05 ) , but the GPx activity and MDA content in gill seemed unable to recover to the level of control group in the experimental period .
出处
《浙江农业学报》
CSCD
北大核心
2016年第7期1148-1155,共8页
Acta Agriculturae Zhejiangensis
基金
河南省重点科技攻关计划项目(072102130027)
河南省高等学校重点科研项目(15B180008)
河南省教育厅自然科学研究计划项目(2011C180009)