硼对黄果柑生理及抗氧化酶活性的影响

Effect of boron on physiological and antioxidant enzymes activity of Huangguogan

以7年生黄果柑果树为材料,设置4个硼处理,研究硼对黄果柑根、叶片生理反应和抗氧化酶活性的影响。结果表明,与对照相比,叶片中不溶性硼含量增长了1-7倍,不溶性硼是根、叶中硼的主要存在形式。硼砂处理显著增加了黄果柑根、叶片中硼和脯氨酸含量,各处理叶片丙二醛含量差异不显著。硼不足和高量都会增大黄果柑果实成熟后期叶片细胞膜透性,显著降低叶片中叶绿素含量,降低果实过氧化氢酶（CAT）、过氧化物酶（POD）和超氧化物歧化酶（SOD）活性。尤其当硼含量较高时,脯氨酸含量有所积累,激活氧化应激反应从而降低黄果柑根、叶的硼毒害作用。

In this study, 7-year-old Huangguogan trees were used as the materials , 4 different boron treatments were set, to study the effect of boron on physiological and antioxidant enzymes activities in Huangguogan roots and leaves . The results showed that the insoluble boron content was increased by 1-7 folds in Huangguogan leaves compared with the control .Insoluble boron was the main form in the roots and leaves .The contents of proline and boron were significantly increased by boron treatments in Huangguogan roots and leaves .There was no significant difference in malondialdehyde （ MDA） content in leaves .The lower and higher concentrations of boron could significantly reduce the chlorophyll content , decrease CAT , POD and SOD activities , and increase cell membrane permeability of the leaves in the late fructescence .When boron content was higher , the content of proline was accumulated , which could activate the oxidative stress reaction , and result in reduction of boron toxicity in Huangguogan roots and leaves .