摘要
为快速、准确、稳定的鉴定南方、花生和爪哇根结线虫,利用已报道的南方和爪哇根结线虫的两对特异性引物,结合本研究设计的花生根结线虫特异性引物,通过优化PCR反应体系,建立了3种根结线虫的PCR检测方法。结果表明,该方法能够特异性扩增以上3种根结线虫,特征片段长度分别为399、335和670 bp,灵敏度达到单条2龄幼虫的水平。研究结果将为以上3种根结线虫的快速鉴定提供技术支持。
To identify Meloidogyne incognita,M. arenaria and M. javanica with rapid,accurate and stable method,two pairs of specific primers for M. javanica and M. incognita from previous studies were chosen,combined with a pair of specific primers for M. arenaria designed in this study,a PCR method was constructed by optimizing PCR reaction parameters. The results showed that the above three Meloidogyne species could be identified with the present method,with characteristic amplicon length of 399,335 and670 bp for M. incognita,M. arenaria and M. javanica,respectively. The sensitivity of this method is one single second-stage juvenile. Results of this study will provide techinical support for the identification of three above-mentioned root-knot nematodes.
出处
《植物检疫》
北大核心
2016年第3期42-47,共6页
Plant Quarantine
基金
国家质检总局课题(2011IK176)
国家科技支撑计划(2012BAK11B03)
宁波市科技创新团队(2015C110018)
天津出入境检验检疫局科技项目(TK072-2013)
关键词
根结线虫
南方根结线虫
花生根结线虫
爪哇根结线虫
PCR
2龄幼虫
分子鉴定
Meloidogyne
Meloidogyne incognita
Meloidogyne arenaria
Meloidogyne javanica
PCR
second-stage juvenile
molecular identification
