MBP在小鼠肝癌H22荷瘤模型中的抗肿瘤作用

Anti-tumor effect of MBP in murine H22 hepatocarcinoma model

目的：探讨大肠杆菌麦芽糖结合蛋白（MBP）在小鼠肝癌H22荷瘤模型中的抗肿瘤作用及可能机制。方法：将12只6～8周龄的BALB/c小鼠皮下注射2×10^6个H22细胞,建立荷瘤模型,荷瘤后第2天将小鼠分为PBS组和MBP组,每组6只,并通过皮下瘤旁注射给药的方式进行干预,期间监测肿瘤体积和小鼠体重变化,测量肿瘤和各脏器质量,ELISA法检测脾细胞分泌IL-12和IFN-γ的水平,Real-time PCR检测肿瘤组织中IFN-γ和IL-17A mRNA的相对表达量。结果：MBP能抑制肿瘤的生长（P〈0.05）。与PBS组相比,MBP组小鼠脾细胞中IL-12、IFN-γ分泌量上升,肿瘤组织中IFN-γmRNA的表达量上升,IL-17A mRNA的表达量下降（P〈0.05）。结论：MBP能够有效抑制肝癌H22肿瘤生长,其作用可能是通过激活Th1型免疫反应和抑制Th17型免疫反应。

Aim： To explore the antitumor efficacy and the mechanism of maltose-binding protein（ MBP） in murine H22 hepatocarcinoma model. Methods： A total of 2 × 10^6H22 hepatoma cells were subcutaneously injected into the flank of twelve female 6- 8 weeks BALB/c mice,respectively. The next day,mice were divided into two groups and treated with PBS or MBP,respectively. Tumor growth and body weight were monitored every day. Tumor and visceral organs were harvested,then tumor weight and visceral index were measured. The level of IL-12 and IFN-γ in supernatant of splenocytes was detected by ELISA assay,and the mRNA expressions of IFN-γ and IL-17 A in tumor tissue was determined using Real-time PCR. Results： Compared with PBS group,MBP significantly suppressed tumor growth（ P 〈0. 05）. Compared with PBS group,splenocytes of MBP group secreted the relative higher levels of IL-12 and IFN-γ,IFN-γ mRNA expression in tumor tissue increased,and IL-17 A mRNA expression in tumor tissue decreased（ P 〈0. 05）. Conclusion： The MBP has antitumor potential,which may be explained by its effects on activating Th1 immune response and inhibiting Th17 immune response.