沉默NOX1表达对A549细胞增殖的影响

Effect of silencing NOX1 on proliferation of A549 cells

目的:探讨NOX1对肺癌A549细胞凋亡和增殖的影响。方法:通过瞬时转染技术转染特异性的NOX1小干扰RNA(siRNA),按转染情况分为Mock组、NC组、FAM-NC-NOX1 siRNA组,转染12 h后采用Western blot检测NOX1蛋白的表达,通过荧光探针DCFH-DA检测细胞ROS水平,流式细胞仪检测细胞增殖活性和凋亡率。结果:siRNA转染12 h以上,转染率稳定在80%以上,NOX1蛋白的表达量与Mock组相比下降(P<0.05);与Mock组相比,FAM-NC-NOX1 siRNA组细胞在24、36、48 h的增殖活性均下降,ROS水平也下降(P<0.05)。结论:NOX1siRNA转染A549细胞能有效抑制细胞的增殖。

Aim： To explore the proliferation and apoptosis in A549 cells after down-regulating NOX1 expression.Methods： NOX1 interference in A549 cells was achieved by NOX1 small interference RNA（ siRNA）. A549 cells were allocated into Mock group,NC group,and FAM-NC-NOX1 siRNA group. Being transfected specifically through the transient transfection technology about 12 h,the expression of NOX1 protein was detected by Western blot,then the fluorescent probe DCFH-DA was used to detect the ROS levels of cells,the flow cytometry was used to detect the changes of cell proliferation activity and apoptosis. Results： The transfection efficiency of NOX1 siRNA was above 80% in A549 cells. Compared with the Mock group,NOX1 protein expression was decreased,the cell viability was decreased at 24,36,48 h after transfection,and the ROS level in FAM-NC-NOX1 siRNA group was decreased（ P〈0. 05）. Conclusion： NOX siRNA transfection could effectively inhibit the proliferation of A549 cells.