呼吸道病毒抗原检测的临床研究

Clinical study of detection of respiratory viruses antigen

目的:探讨呼吸道病毒抗原检测在婴幼儿上呼吸道感染性疾病诊断中的应用价值。方法:采用直接荧光免疫法对我院收治的600例上呼吸道感染患儿进行呼吸道合胞病毒、腺病毒、副流感Ⅰ型病毒、副流感Ⅱ型病毒、副流感Ⅲ型病毒、流感A型病毒以及流感B型病毒(简称7项呼吸道病毒)检测,同时进行以上病毒相关血清Ig M抗体检测。结果:在600例上呼吸道感染性疾病中有134例呼吸道病毒抗原检测为阳性,占上呼吸道感染患儿的22.33%;病毒Ig M抗体阳性98例,占上呼吸道感染患儿的16.33%,二者阳性检出率差异有统计学意义(P<0.05)。结论:呼吸道病毒抗原检测有助于提高对呼吸道病毒感染的诊断率,方便临床及时制定合理的治疗方案。

Objective： To investigate the value of clinical application of respiratory virus antigen detection for diagnosis of respiratory tract infections in infants and young children. Methods： 600 case of children with respiratory tract infection hospitalized in our hospital were detected for respiratory syncytial virus,adenovirus, vice influenza Ⅰ type virus, vice influenza Ⅱ type virus, vice influenza Ⅲ type virus,influenza A virus and influenza B type virus（hereinafter referred to as the seven respiratory virus） by direct immunofluorescence method and serum Ig M were also detected. Results： In 600 case of children with upper respiratory tract infection diseases, the detection of respiratory viruses antigen was positive for134 children, accounting for 22.33% of the upper respiratory tract infections, 98 children were detected by Ig M antibody to positive, accounting for 16.33% of the children with upper respiratory tract infections. The difference of the two positive detection rate was statistically significant（P 0.05）. Conclusion： The diagnosis rate of respiratory viruses infection can be improved by the detection of respiratory viruses antigen, and it is convenient for clinical treatment to be reasonable.