喉癌组织RKIP基因甲基化表达调控研究

Study on expression and regulation of RKIP gene methylation in laryngeal carcinoma

目的检测喉癌组织中Raf-1激酶抑制蛋白(Raf-1 kinase inhibitor protein,RKIP)基因启动子区域的甲基化状态及其蛋白表达情况,并探讨其临床意义。方法采用甲基化特异性PCR(methylation-specific PCR,MSP)技术及免疫组化方法检测53例喉癌组织及34例癌旁组织中RKIP基因启动子区域的甲基化状态及蛋白表达情况。结果喉癌及癌旁组织中RKIP基因启动子甲基化发生率分别为17%、3%,两者比较差异具有统计学意义(P=0.045)。结论 RKIP基因启动子区高甲基化状态可能是造成喉癌组织中RKIP表达缺失的分子机制之一,RKIP蛋白表达缺失与喉癌的发生发展及转移有关。

Objective To investigate methylation status in promoter region of raf-1 kinase inhibitor protein（RKIP） gene and the expression of RKIP protein in laryngeal carcinoma, and to discuss their clinical significances. Methods Methylation- specific PCR and immunohistochemistry were used to examine the methylation status of RKIP gene promoter region and the expression of RKIP protein in 53 laryngeal carcinoma tissues and 53 normal adjacent tissues. Results The incidences of RKIP gene promoter methylation in laryngeal carcinoma and adjacent normal tissues were 17% and 3% respectively. The difference was statistically significant （P = 0. 045）. Conclusion Promoter hypermethylation of RKIP may be one reason of the deleted expression of RKIP protein, which correlates with occurrence, development and metastasis of laryngeal carcinoma.