柴胡皂苷d治疗咳嗽变异性哮喘的实验研究

Effect of saikosaponin-d in treatment of cough variant asthma: an experimental study

目的:观察柴胡皂苷d对咳嗽变异性哮喘(CVA)豚鼠的治疗作用,并探究其作用机制。方法:将32只豚鼠随机分成正常组、模型组、柴胡皂苷d组和激素组,采用综合法复制CVA豚鼠模型,并给予相应药物干预,观察豚鼠体征指标、咳嗽次数、支气管肺泡灌洗液嗜酸性粒细胞计数,检测血清IL-5和TNF-α水平的变化。结果:与正常组比较,模型组豚鼠均出现阵发性咳嗽、喷嚏、呼吸急促、躁动不安等表现,豚鼠咳嗽次数明显增加(P<0.01),嗜酸性粒细胞明显增多(P<0.01),血清IL-5和TNF-α水平均明显增高(P<0.05);给予相应药物干预之后,柴胡皂苷d组和激素组豚鼠咳嗽次数明显减少,嗜酸性粒细胞明显减少,血清IL-5和TNF-α水平均明显降低,与模型组比较,差异均有统计学意义(P<0.05,P<0.01)。结论:柴胡皂苷d可能通过降低CVA豚鼠IL-5和TNF-α的表达,达到治疗CVA的目的。

Objective： To investigate the therapeutic effect of saikosaponin- d in guinea pigs with cough variant asthma（ CVA） and related mechanisms of action. Methods： A total of 32 guinea pigs were randomly divided into normal group,model group,saikosaponin- d group,and hormone group. The comprehensive method was used to establish the guinea pig model of CVA and the corresponding drug intervention was given. The signs,times of cough,and eosinophil count in bronchoalveolar lavage fluid were observed in all guinea pigs,and the changes in the serum levels of interleukin- 5（ IL- 5） and tumor necrosis factor- α（ TNF- α） were determined. Results： Compared with the normal group,the model group experienced paroxysmal cough,sneeze,tachypnea,and restlessness / uneasiness and showed significant increases in the times of cough（ P〈0. 01）,eosinophil count（ P〈0. 01）,and serum levels of IL- 5 and TNF- α（ P〈0. 05）. After the corresponding drug intervention was given,the saikosaponin- d group and the hormone group showed significant reductions in the times of cough,eosinophil count,and serum levels of IL- 5 and TNF- α（ P〈0. 05,P〈0. 01）. Conclusion： Saikosaponin- d can reduce the expression of IL- 5 and TNF- α in guinea pigs and thus achieve the goal of treating CVA.