液相色谱-质谱法快速测定小儿清解颗粒中非法添加的氨基比林

Liquid Chromatogram and Mass-spectrography for Rapid Determination of Illegally Added Amidopyrine in Pediatric Qingjie Particles

目的:建立快速准确鉴定小儿清解颗粒中非法添加氨基比林的方法。方法:用液相色谱质谱联用技术对4种不同厂家小儿清解颗粒中非法添加消肿止痛类化学药品氨基比林进行筛查,与氨基比林对照品的一、二级质谱图进行比较。结果:在样品质谱图中有3家样品的图谱与对照品有不一致的一级质谱峰与二级质谱峰,1个厂家的样品图谱有与对照品有一致的一级质谱峰与二级质谱峰。结论:应用液相色谱-质谱法可快速准确鉴定出中药制剂中是否含有非法添加的氨基比林,并可以准确计算添加氨基比林的含量。

OBJECTIVE：To establish a rapid accurate identification method of illegally added amidopyrine in pediatric Qingjie particles .METHODS：Illegally added acetanilide detumescence chemical drug amidopyrine from four different companies were screened by liquid chromatogram （ LC ） and mass-spectrography （ MS ） , and reference substance of secondary mass spectra were compared .RESULTS： In the sample mass spectrum , there were three samples were not consistent with the reference substance of primary and secondary mass spectrum peak , and a manufacturer of sample pattern was consistent with the reference substance of primary and secondary mass spectrum peak.CONCLUSIONS： Application of LC and MS method can accurately identify whether Chinese medicine preparations contains illegal added amidopyrine , and the content of aminopyrine can be calculated accurately .