全反式维甲酸联合辛二酰苯胺异羟肟酸诱导MCF-7细胞凋亡

All Trans Retinoic Acid Combined with Suberoylanilide Hydroxamic Acid Induced Apoptosis of MCF-7 Cells

目的:探讨全反式维甲酸(ATRA)和辛二酰苯胺异羟肟酸(SAHA)单独及联合应用对乳腺癌MCF-7细胞凋亡的影响。方法:培养人乳腺癌MCF-7细胞,待细胞进入对数生长期,加入一定浓度的ATRA和(或)SAHA,采用流式细胞术检测细胞的凋亡情况。结果:ATRA和SAHA单药及联合应用早期凋亡率均高于对照组,但24 h时测得联合组早期凋亡率低于单药组,48 h、72 h联合组早期凋亡率高于SAHA组却低于ATRA组,差异均有统计学意义(P<0.05);联合组死亡细胞和晚期凋亡细胞率之和高于单药组及对照组,差异有统计学意义(P<0.05)。结论:ATRA和SAHA单独或联合作用于乳腺癌MCF-7细胞均可促进细胞凋亡,两药联合应用时诱导细胞凋亡作用更强。

Objective： To investigate the effects of all trans retinoic acid （ATRA） and suberoylanilide hydroxamic acid （SAHA） on the apoptosis of MCF-7 and to investigate whether the combination of the two drugs have synergistic effect. Methods： Human breast cancer eeU line MCF-7 was cultured in vitro. When the cells entered the logarithmic growth phase, a certain concentration of ATRA （or） SAHA was added into the petri dishes. Flow cytometry was used to detect the apoptosis of the cells. Results： Compared with the control group, the early apoptosis rate was higher in the single drug groups and combined group. But the early apoptosis rate of the combined group was lower than that of the single drug group at 24 h, and the early apoptosis rate of 48 h and 72 h in combined group was higher than that in ATRA group but lower than SAHA group. The difference was statistically significant （P〈0.05）. The rate of death and late apoptotic cells in the combination group was higher than that in the single drug groups and the control group, the difference was statistically significant （P〈0.05）. Conclusions： ATRA and SAHA can promote the apoptosis of breast cancer cells ATRA combined with SAHA induced apoptosis of cells is more intense than the single drug groups.