运动预干预通过增强杏仁核抗氧化能力及BDNF表达改善睡眠剥夺大鼠焦虑行为

Prior Exercise Impairments Anxiety-Like Behavior and Increase in Amygdala Antioxidant Capacity and BDNF Expression in Sleep Deprived Rats

目的:研究表明体育锻炼可以预防睡眠不足引起的焦虑行为,然而机制尚不清楚。因此,通过探讨4周跑台运动预干预对睡眠剥夺大鼠焦虑样行为的影响,并通过检测杏仁核氧化应激水平及BDNF的表达,探讨跑台运动影响睡眠剥夺大鼠焦虑样行为的可能机制。方法:实验前将大鼠随机分为安静组(S,n=10)、运动组(E,n=10)、安静睡眠剥夺组(SSD,n=10)和睡眠剥夺运动组(ESD,n=10)。除S及SSD组大鼠外,E及ESD组大鼠进行中等强度跑台运动预干预4周,随后采用小平台水环境法建立大鼠72h睡眠剥夺模型,然后运用高架十字迷宫实验评估大鼠焦虑样行为。焦虑样行为学测试结束后,检测大鼠杏仁核超氧化物歧化酶(SOD)及过氧化氢酶(CAT)的活性,计算杏仁核BDNF的表达。结果:与安静组比较,高架迷宫实验中,72 h睡眠剥夺导致大鼠闭合臂时间百分比CT%(P<0.01)及粪便颗粒(P<0.05)增多、(OE+CE)次数(P<0.01)及开放臂时间百分比OT%(P<0.01)均显著减少,杏仁核SOD、CAT活性(P<0.01,P<0.01)及BDNF表达均显著下降(P<0.01)。而适度的跑台运动预干预可削弱睡眠剥夺引起的焦虑行为,杏仁核BDNF表达显著提升(P<0.05),抗氧化酶SOD及CAT的活性增强(P<0.05,P<0.05)。结论:规律跑台运动预干预可以通过提高睡眠剥夺大鼠杏仁核BDNF表达及抗氧化能力,对杏仁核相关功能产生保护作用,削弱睡眠剥夺大鼠的焦虑样行为。

Objetive： Evidence suggests that treadmill exercise can protect sleep deprivation induced anxiety-like behavior.However the mechanism is unclear. So we examined the impact of 4 weeks of prior regular treadmill exercise on sleep deprivation induced anxiety-like behavior and possible mechanism by detecting oxidative stress level and calculating BDNF expression of the rat amygdala. Methods： The rats were randomly divided into the follow ing subgroups： the sedentary group（ S）,the exercise group（ E）,sedentary sleep deprived group（ SSD） and exercise sleep-deprived group（ ESD）. E and ESD rats were exposed to 4 weeks of regular treadmill exercise,and then subsequently were deprived of sleep for 72 h using small platform method. Then elevated plus-maze test（ EPM） were used to evaluate the anxiety-like behavior of rats; antioxidant enzymes SOD and CAT activities in amygdala were examined and amygdala BDNF expression was calculated.Results： Compared with S group,CT% and fecal particles increased（ P〈0.01,P〈0.05）,（OE＋CE） numbers（ P〈0.01） and OT%（ P〈0.01） significantly decreased in EPMtest of SSD group rats; SOD and CAT activities and BDNF expression significantly reduced after 72 h sleep deprivation in SSD rats compared with the S groups. Results showed that sleep- deprived rats under moderate treadmill significantly attenuated sleep deprivation induced anxiety-like behavior,BDNF expression had a significant up-regulation（ P〈0.05） and SOD,CAT activities in amygdala significantly enhanced（ P〈0.05,P〈0.05）. Conclusion： prior regular exercise can exert a protective effect on amygdala-related functions and impairments anxiety-like behavior induced by sleep deprivation probably by inducing BDNF expression and decreasing oxidative damage in amygdala.