急性胰腺炎相关性肺损伤小鼠肺组织蛋白组学初步研究

Proteomics analysis of acute pancreatitis-associated lung injury in mice

目的应用蛋白质组学方法筛选急性胰腺炎相关性肺损伤(APALI)小鼠模型与正常小鼠肺组织蛋白质表达差异,为阐明APALI的发病机制和预后提供理论依据。方法 20只BALB/c小鼠随机分为实验组和对照组,实验组通过腹腔注射雨蛙肽的方法造模,造模后处死小鼠取左肺组织进行病理学分析,右肺组织提取肺组织总蛋白,用双向电泳加质谱的技术路线进行蛋白质组学分析。结果分析差异蛋白质功能,发现差异蛋白质主要与炎症反应及氧化还原相关。结论炎症浸润、组织细胞损伤引起的氧化应激损伤可能在APALI发病早期扮演重要角色,在APALI早期重视抗氧化治疗可有助于改善预后。

Objective To screen the differentially expressed proteins of lung tissue between acute pancreatitis-associated lung injury （APALI） mice and wild type （wt） mice by using proteomics methods, which may provide an evidence for study of the pathogenesis and prognosis of APALI. Methods 20 BALB/ c mice were randomly divided into the experimental and control groups. Caerulein was injected intraperitonealy to establish the APALI model. Mice were sacrificed after one day of caerulein injection and left lung was collected for pathology examination. Total proteins from right lung were extracted for 2-DE and proteomics research. The differentially expressed protein spots were identified to explicit the biological functions. Results The lung-injury symptoms were observed in experimental mice. The differentially expressed proteins were closely related to inflammatory and redox reactions. Conclusion Oxydative stress induced by inflammatory infiltration and tissue injury is crucial in the early development of APALI. Antioxydant treatment in the early stage may be helpful for prognosis of APALI.