免疫磁纳米粒子对肠炎沙门氏菌的富集分离

Enrichment and Isolation of Salmonella enteritidis by Immune Magnetic Nanoparticles

采用化学共沉淀法制备了羧基化磁纳米粒子,分别对磁纳米粒子-沙门氏菌多克隆抗体复合物(免疫磁纳米粒子)的偶合条件和免疫磁纳米粒子富集分离肠炎沙门氏菌的条件进行了优化,为肠炎沙门氏菌的富集分离和检测提供一种更为快捷、高效的方法。结果表明,当51.7μg/m L羧基化磁纳米粒子与碳二亚胺/N-羟基丁二酰亚胺(0.4 mol/L/0.1 mol/L)、1.0 mg/m L的多克隆抗体的体积比为1∶2∶2时,37℃水浴加热40 min,两者的偶合效果最佳。应用上述优化条件制备的免疫磁纳米粒子吸附104 CFU/m L肠炎沙门氏菌,当两者的体积比为4∶5,孵育时间为40 min时,免疫磁纳米粒子对肠炎沙门氏菌的吸附效率可达到94.36%。

In this study, carboxyl-coated magnetic namoparticles（MNPs） were prepared using a chemical coprecipitation method. The coupling conditions for conjugates（immune MNPs） of MNPs and anti-Salmonella polyclonal antibody（PAb） were optimized, and then the application of immune MNPs in the enrichment and isolation of Salmonella enteritidis was evaluated, which will provide a more fast and effective way for the detection of Salmonella. The results showed that the optimal coupling of MNPs and PAb was achieved when the volume ratio of 51.7 μg/m L MNPs to 0.4 mol/L EDC-0.1 mol/L NHS to 1.0 mg/m L PAb was 1：2：2 in a water bath at 37 ℃ for 40 min. The adsorption efficiency attained 94.36% when the volume ratio between 51.7 μg/m L immune MNPs and 104 CFU/m L S. enteritidis was 4：5, and the incubation time was 40 min.