双歧杆菌BB12对便秘大鼠的通便作用及对胃肠激素的调节

Bifidobacterium BB12 Promotes Defecation and Regulates Gastrointestinal Hormones in Constipated Rats

目的:研究双歧杆菌BB12对便秘大鼠的通便作用及对相关胃肠激素的调节。方法:将48只Wistar大鼠按性别分开,雌雄各半。按体质量随机分为正常对照组、阳性对照组、模型对照组、双歧杆菌BB12低、中、高剂量组,每组4只。造模后灌胃相应受试样品,记录各组大鼠每日进食量、饮水量、体质量、排便粒数,测定粪便含水量、小肠炭末推进率、血清胃泌素、胃动素、P物质、生长抑素含量。结果:双歧杆菌BB12能显著减少便秘大鼠的饮水量,增加便秘大鼠的排便粒数、小肠炭末推进率、血清胃动素和P物质含量,降低生长抑素含量,且作用具有一定的性别差异和剂量相关性。对便秘大鼠的进食量、体质量、粪便含水量、血清胃泌素含量无明显作用。结论:双歧杆菌BB12对便秘大鼠具有通便作用,其作用机制与其调节胃肠激素的分泌有关。

Objective： To investigate the effects of Bifidobacterium BB12 on promoting defecation and regulatinggastrointestinal hormones in constipated rats. Methods： Totally 48 Wistar rats, half male and half female, were dividedrandomly into control group, positive control group, model group, Bifidobacterium BB12 low-, middle-, and high-dosegroups. Rat models of loperamide hydrochloride-induced constipation were established and the rats from each group weretreated with corresponding drugs. During the experiment, daily food intake, water intake, body weight, fecal number, fecalwater content, gastrointestinal transit, and the contents of gastrin, motilin, substance P and somatostatin in serum weremeasured. Results： Fecal number, gastrointestinal transit, and the serum contents of motilin and substance P increasedsignificantly, while water intake and somatostatin content decreased significantly in response to the administration ofBifidobacterium BB12. These effects were dependent on gender and dose. There was no significant difference in food intake,body weight, fecal water content and serum gastrin. Conclusion： The laxative effect of Bifidobacterium BB12 is confirmedand the underlying mechanism is related to regulating some gastrointestinal hormones.