湿热处理多孔玉米淀粉对大鼠脂质代谢的影响

Effect of Heat-Moisture Treated Corn Porous Starch on Lipid Metabolism in Rats

目的:探讨湿热处理多孔玉米淀粉对大鼠脂质代谢的影响。方法:32只雌性Sprague-Dawley(SD)大鼠随机分为4组,其中1组为对照组饲喂基础饲料,另外3组为高脂组,分别为高脂空白组、多孔淀粉组(porous?starch,PS)和湿热处理多孔淀粉组(heat?moisture?treatment?starch,HTMPS),饲喂基础饲料,适应1周后,对照组饲喂基础饲料,高脂组分别饲喂相应高脂饲料。饲喂4周后解剖,检测大鼠血脂、肝脂及小肠和粪便中胆汁酸等指标。结果:饲喂多孔淀粉和湿热处理多孔淀粉组大鼠血清总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、非高密度脂蛋白胆固醇(non-high-density lipoprotein cholesterol,non-HDL-C)、抗动脉硬化指数(atherosclerosis index,AI)、甘油三酯/高密度脂蛋白胆固醇(TG/HDL-C)、肝脏TC、肝脏总TC和腹部脂肪含量显著下降(P<0.05),粪便中粪醇、胆固醇等中性固醇排泄量增加,粪便中胆汁酸排泄量显著增加(P<0.05)。PS组与HTMPS组相比,大鼠血浆中TC、AI、腹部脂肪含量、肝脏总脂肪含量较低,粪便中胆汁酸及小肠内容物中胆汁酸较高。结论:多孔淀粉降血脂降胆固醇效果优于湿热处理多孔玉米淀粉,可降低高脂饮食造成的高脂血症风险,对预防心血管疾病有一定的作用。

Objective： To investigate the effect of heat-moisture treated corn porous starch on lipid metabolism in rats.Methods： Totally 32 female Sprague-Dawley rats were randomly divided into 4 groups including control group and threeother groups as high-fat blank group, high-fat porous starch （PS） group and high-fat heat-moisture treated corn porous starch（HTMPS） group. After adaptive feeding with a basal diet for a week, the rats from the control group were still fed with thebasal diet and those from high-fat groups were given a high fat diet. After 4 weeks, all rats were sacrificed for the analysisof triglyceride （TG）, cholesterol （TC） in serum and liver, and bile acid in small intestine and feces. Results： By feeding cornporous starch and heat-moisture treated corn porous starch, the contents of TC, TG and non-HDL-C in serum, atherosclerosisindex （AI） and TG/HDL-C ratio in serum as well as TC content in liver and abdominal fat content decreased significantly（P 〈 0.05）; the fecal excretion of neutral steroid and bile acid increased significantly （P 〈 0.05）. Compared with HTMPSgroup, the rats from PS group revealed a reduction in serum TC level, AI, TG/HDL-C ratio and abdominal fat, and anincrease in fecal bile acid excretion and bile acid in small intestinal contents. Conclusion： PS is more successful in reducingcholesterol than HTMPS. Therefore, porous starch can reduce the risk of hyperlipidemia caused by high-fat diet, and has asignificant role in preventing cardiovascular diseases.