摘要
将牛免疫球蛋白G（immunoglobulin G,Ig G）作为免疫原免疫BALB/c小鼠,通过细胞融合、筛选获得分泌抗牛Ig G单克隆抗体的细胞株。制备小鼠腹水抗体,进一步纯化获得抗牛Ig G单克隆抗体。建立双抗夹心酶联免疫吸附法检测牛初乳中Ig G质量浓度,该方法在7.8~1 000 ng/m L范围内有良好的线性关系,最低检出限为7.06 ng/m L,批内变异系数为4.52%,批间变异系数为4.94%,回收率为91.85%~102.45%。此法操作简便、准确度高、稳定性好,可用于实际牛初乳样品的快速检测。
The hybridoma cells secreting monoclonal antibody（Mc Ab） against bovine colostrum immunoglobulin G（Ig G） were developed by cell fusion technology after immunization of BALB/c mice with bovine Ig G. Mc Abs were obtained by purification of mouse ascites. A double antibody sandwich enzyme-linked immunosorbent assay（ELISA） technique was established for the detection of bovine colostrum Ig G. The linear range was 7.8–1 000 ng/m L and the limit of detection（LOD） was 7.06 ng/m L. The intra-assay and inter-assay coefficient of variations were 4.52% and 4.94%, respectively. The recovery rate was 91.85%–102.45%. The ELISA method can provide a simple, accurate and stabile approach for the rapid detection of bovine colostrum Ig G.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第14期74-79,共6页
Food Science
基金
2011年度国家星火计划项目(2011GA650001)
