高效液相色谱法同时测定降脂颗粒中虎杖苷、二苯乙烯苷、橙皮苷含量

Simultaneous Content Determination of Polydatin,Styrene Glycosidesand and Hesperidin in Jiangzhi Granules by HPLC

目的建立测定降脂颗粒中的虎杖苷、二苯乙烯苷、橙皮苷含量的高效液相色谱法。方法色谱柱为Kromasil C18柱（250 mm×4.6 mm,5μm）,流动相为乙腈-水（20∶80）,检测波长283 nm、320 nm,柱温30℃,流速1.0 m L/min。结果虎杖苷质量浓度在5.12~38.40μg/m L范围内与峰面积有良好线性关系（r=1.000）,平均回收率为100.13%,RSD为0.63%（n=6）;二苯乙烯苷质量浓度在1.91~19.09μg/m L范围内与峰面积有良好线性关系（r=1.000）,平均回收率为99.14%,RSD为0.63%（n=6）;橙皮苷质量浓度在9.89~74.19μg/m L范围内与峰面积有良好线性关系（r=1.000）,平均回收率为99.54%,RSD为0.31%（n=6）。结论该法灵敏、简便、准确,重现性好,线性关系良好,可作为制剂质量控制的标准。

Objective To establish an HPLC method for the content determination of giant knotweed polydatin, styrene glycosides （ TSG ） and hesperidin in Jiangzhi Granules. Methods Kromasil C18 （ 250 mm × 4. 6 mm, 5 μm ） column was used with acetonitrile-water （ 20 ：80 ） as mobile phase;the detecting wavelength：283 nm and 320 nm. The column temperature was 30 ℃, the flow rate was 1. 0 mL /min. Results Polydatin, TSG and hesperidin in the concentration ranges of 5. 12-38. 40 μg/mL （ r=1. 000 ） , 1. 91~19. 09 μg/mL （ r=1. 000 ） , and 9. 89-74. 19 μg/mL （ r=1. 000 ） , respectively, exhibited a good linear relationship with the peak area. The average recovery rates of the above 3 compounds were 100. 13%, RSD=0. 63% （ n=6 ）;99. 14%, RSD=0. 63% （ n=6 ）;99. 54%, RSD=0. 31% （ n=6 ） . Conclusion This method is sensitive, simple and accurate with good reproducibility and fine linear relationship, and can be used for the quality control of said preparation.