摘要
CHO细胞表达系统是目前重组糖蛋白生产的首选系统。随着无血清悬浮培养技术、基因工程技术和大规模培养技术的应用和不断发展,CHO细胞表达系统已经成为生物技术药物最重要的表达或生产系统,并被广泛应用于抗体、重组蛋白药物和疫苗等产品的研发和生产中。近年来,针对CHO细胞表达系统在某些重组蛋白的表达和大规模生产中存在的不足,研究者们通过利用基因工程技术手段,结合重组蛋白表达机制的研究成果,为优化和应用CHO细胞表达系统做出了不懈努力。从培养基的优化、高产重组CHO细胞株的构建、大规模培养三个方面综述了CHO细胞表达系统的最近研究进展,以期为CHO细胞表达系统的研究与应用提供参考。
CHO cell expression system is the preferred system for recombinant glycoprotein production. With the evolving development and applications of serum- free suspension culture technology,genetic engineering and the large-scale culture technologies,CHO cell expression system has become the most important expression or production system of biotechnology products. This system is widely used in the research and production of antibodies,recombinant proteins and vaccines. In recent years,researchers have made great efforts to improve the expression and large-scale production of recombinant proteins by using latest bioengineering technology and the development of the recombinant protein expression mechanism. This article briefly reviewed the recent development of the CHO cell expression system in three aspects： the optimization of the culture medium,construction of engineered CHO strains for high-level production and large-scale culture research,which was expected to provide reference for research and application of CHO cell expression system.
出处
《生物技术进展》
2016年第4期239-243,共5页
Current Biotechnology
关键词
CHO细胞培养
细胞改造
重组抗原表达
CHO cell culture
cell engineering
recombinant antigen expression
