糖蛋白药物的多糖结构解析进展

Progress on Glycan Analysis of Therapeutic Glycoprotein

细胞表达的治疗性单克隆抗体多数是糖蛋白。附着在蛋白质上的多糖直接影响蛋白质药物的稳定性、生物活性及免疫原性。因此,应对糖蛋白产品上的多糖进行充分分析,以控制产品质量。然而,糖蛋白上多糖的复杂性对检测带了艰巨挑战。介绍了糖蛋白上多糖分析的最新进展,以及利用凝集素芯片技术的高通量多糖分析法,重点介绍了用于检测糖基化位点、糖链结构和含量的测定分析方法,以期为糖蛋白药物的研发提供参考。

Therapeutic monoclonal antibodies（ m Abs） are glycoproteins produced by living cell systems. The glycan moieties attached to the proteins can directly affect protein stability,bioactivity and immunogenicity. Therefore,glycan variants of a glycoprotein product must be adequately analyzed and controlled to ensure product quality. However,the inherent complexity of protein glycosylation poses a daunting analytical challenge. This review provided an update of recent advances in glycan analysis,including the potential utility of lectin-based microarray for high throughput glycan proling. Emphasis was placed on comparison of the major types of analytics for use in determining unique glycan features such as glycosylation site,glycan structure and content,which was expected to provide reference for research of glycoproteins.