微孢子虫感染的柞蚕蛹内细菌种群分布及遗传多样性分析

On Diversity of Bacterial Population in Microsporidia-Effected Pupa of Chinese Oil SilkwormAntherea Pernyi

分离纯化微孢子虫感染的柞蚕蛹内单细胞微生物,采用CTAB法提取混合样品总DNA,并应用高通量SOLEXA测序技术进行测序.通过生物信息学方法进行分析比较,共鉴定了87个种,隶属于37个属17个科的细菌,主要分布在变形杆菌属Proteus、乳球菌属Lactococcus、假单胞菌属Pseudomonas、芽孢杆菌属Bacillus、肠球菌属Enterococcus等,揭示了微孢子虫感染的柞蚕蛹内细菌种群的多样性.对细菌相关基因的COG(同源蛋白簇)注释显示,参与产能及能量代谢的基因数量所占比例最多;对蛋白序列遗传距离的分析显示,编码硫胺素、二硫键分子伴侣、NADH泛醌氧化还原酶NqrA亚基等的基因在细菌种群中遗传变异相对较大,说明在蛹环境中这些参与能量代谢的相关基因变异速度加快.研究感染微孢子的宿主体内细菌物种的多样性,对于进一步挖掘微孢子虫与细菌共寄生宿主过程中的相互作用具有潜在意义.

Microorganism s from the pupa of Chinese oil silkwoxm（Antheraeae p e rn y i） that is effected bym icrosporidia parasite have been isolated and purified, and then high-throughput sequencing of all DNAsfrom com plex m ixtures have been perform ed to survey the population of bacteria that co-infected w ith microsporidiaparasite. Based on data analysis of the assem bled m icrobial genom e sequences in this study,eighty-seven bacterial species have been found in pupa and can classified into 37 genera. T he m ajor bacterialspecies inhabiting the m icrosporidia-effected pupa harbor the genus of P roteus, L actococcus, Pseudomonas , Bacillus, Enterococcus. T he function of the bacterial-related genes are annotated and classifiedthrough searching against COG database. Significantly, the genetic distance of bacterial genes are involvedin the energy production and conversion have been found to be higher than that of other genes, indicatingthat they m ight be fast-evolving genes for the host adaption. O ur results may potentially benefit for thestudy of the interaction of m icrosporidia and bacteria that co-effected in the host.