盐胁迫下旱地棉叶片数字化基因表达谱分析

Digital gene expression profile of Gossypium aridum leaves under salt stress

为了深入研究棉花耐盐的分子机理,利用数字化基因表达谱技术,分析了盐胁迫处理3 h和72 h时旱地棉叶片中基因表达差异,分别获得1 778(1 046个上调、732个下调)和2 873(2 065个上调、808个下调)个差异表达基因。功能注释分析结果表明,这些差异表达基因主要参与了信号转导、逆境应答、能量代谢和转录调节等方面。随机挑选10个差异表达基因进行qRT-PCR验证,结果表明,qRT-PCR结果与数字化基因表达谱分析结果基本一致。

To uncover the salt tolerance mechanisms of Gossypium aridum and improve salinity resistance,the technique of digital gene expression profile was involved to analyze the variations of gene expression in the leaves of upland cotton exposed to 200 mmol/L Na Cl for 3 h and 72 h,respectively. There were 1 778（ 1 046 up-regulated/732 down-regulated） and 2 873（ 2 065 up-regulated/808down-regulated） differentially expressed genes,respectively. Functional annotation analysis revealed that the genes were involved in various metabolic pathways including signal transduction,stress response,energy metabolism,and transcriptional regulation. A total of 10 differentially expressed genes were randomly selected for qRT-PCR validation,and the results were basically consistent with those by the digital gene expression profile.