荷斯坦牛TLR6基因CDS区的生物信息学分析

Bioinformatics analysis of CDS of TLR6 gene in Holstein cattle

采用生物信息学方法分析和预测了荷斯坦牛TLR6基因编码蛋白质的理化特性、结构功能及其同源进化关系。结果表明,荷斯坦牛TLR6基因编码793个氨基酸,组成蛋白为不稳定的水溶性蛋白,二级结构为混合型,三级结构由α-螺旋、β-折叠、β-转角和无规则卷曲组成。蛋白质功能显示信号转导、胁迫应答、免疫应答和生长因子的几率相对较高。荷斯坦牛TLR6基因氨基酸序列与牦牛的同源性较高。说明TLR6基因能在荷斯坦牛免疫应答和生长调节过程中发挥重要作用,可能对牛的抗病力和生产性能起作用。

In order to provide a theoretical basis for disease resistance molecular breeding and genetic screening of economic traits of cattle,the physical and chemical properties,the structure and function of the encoded product and homologous evolutionary relationships of TLR6 gene in Holstein cattle were analyzed and predicted by means of bioinformatics.TLR6 gene encodes 793 amino acids. The TLR6 gene-encoded protein is a kind of instable and soluble protein,with mixedtype secondary structure and tertiary structure consisting of alpha helix,extended strand,beta turn and random coil. The TLR6 protein was predicted to play a major role in signal transduction,stress response,immune response and growth factor.The amino acid sequence of Holstein cattle TLR6 shared high homology with that of yaks. Due to its role in the immune response and growth adjustment process,TLR6 gene may be responsible for disease resistance and production performance of Holstein cattle.